characteristics.text: https://github.com/AusMicrobiome/contextualdb_doc/raw/4.2.0/db_schema_definitions/db_schema_definitions.xlsx
characteristics.characteristic: AustralianMicrobiome_metadata_defs_url
characteristics.text: SAMN43264581
characteristics.characteristic: External Id
characteristics.text: CSIRO
characteristics.characteristic: INSDC center name
characteristics.text: 2024-08-19T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-08-20T00:48:25.743Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS22400535
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: MIMS.me
characteristics.characteristic: NCBI submission model
characteristics.text: MIGS/MIMS/MIMARKS.sediment
characteristics.characteristic: NCBI submission model
characteristics.text: MIMS.me.sediment.6.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS22400535
characteristics.characteristic: SRA accession
characteristics.text: 2.632
characteristics.characteristic: absorbance_260_280_ratio_submitter
characteristics.text: meth_NanoPhotometer NP80
characteristics.characteristic: absorbance_260_280_ratio_submitter_meth
characteristics.text: 2020-11-01T13:00:00Z
characteristics.characteristic: collection_date
characteristics.text: 0 m - 0.01 m
characteristics.characteristic: depth
characteristics.text: Keywords: GSC:MIxS;MIMS:6.0
characteristics.characteristic: description
characteristics.text: 0.01 ng/μL
characteristics.characteristic: dna_concentration_submitter
characteristics.text: meth_NanoPhotometer NP80
characteristics.characteristic: dna_concentration_submitter_meth
characteristics.text: not collected
characteristics.characteristic: elev
characteristics.text: 6 Water
characteristics.characteristic: env_broad_scale
characteristics.text: 6.6 Estuary/coastal waters
characteristics.characteristic: env_local_scale
characteristics.text: Sediment [ENVO_00002007]
characteristics.characteristic: env_medium
characteristics.text: 5.20583414760671 %
characteristics.characteristic: fine_sediment
characteristics.text: meth_1. Transfer sediments into pre-weighed labelled aluminium trays. 2. Place in drying oven at 60°C for 24hrs. 3. Using a sediment shaker for 1 min; shake sample through 4mm; 2mm; 1mm; 500um; 250um; 125um and 63um size sieves. 4. Transfer sediments retained on each sieve into pre-weighed weighing boats. 5. Weigh each fraction and calculate % fines.
characteristics.characteristic: fine_sediment_meth
characteristics.text: Australian Microbiome Initiative
characteristics.characteristic: funding_agency
characteristics.text: Coastal (coastal terrestrial including cliff; rocky shore)
characteristics.characteristic: general_env_feature
characteristics.text: Australia:New South Wales [GAZ_00002793]
characteristics.characteristic: geo_loc_name
characteristics.text: 102.100.100/401030_0
characteristics.characteristic: isolate
characteristics.text: sediment
characteristics.characteristic: isolation_source
characteristics.text: 33.142324 S 151.591711 E
characteristics.characteristic: lat_lon
characteristics.text: 749907
characteristics.characteristic: ncbi_txid
characteristics.text: Qiagen PowerSoil Kit
characteristics.characteristic: nucl_acid_ext
characteristics.text: 0.678855203270911 %
characteristics.characteristic: org_matter
characteristics.text: meth_1. Transfer sediment to pre-weighed labelled aluminium tray. 2. Dry in oven at 60°C for 24hrs. 3. Grind to a fine powder using a mixing mill (MM200). 4. Place samples in pre-weighed labelled crucibles and transfer to muffle furnace at 105°C for 24 hrs. 5. Remove samples; allow to cool and weigh. 6.Return crucibles to muffle furnace at 430°C for a further 24 hours. 7. Remove samples; cool and weigh. Calculate % organic matter.
characteristics.characteristic: org_matter_meth
characteristics.text: sediment metagenome
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_749907
characteristics.characteristic: organism
characteristics.text: meth_Sterile 15ml tube
characteristics.characteristic: samp_collect_device
characteristics.text: Samples frozen at -80°C until DNA extraction. For DNA extraction samples were defrosted and a spatula (sterilised with 10% bleach) was used to weigh 0.25g of sediment into bead beating tubes.
characteristics.characteristic: samp_mat_process
characteristics.text: Alessandra Suzzi
characteristics.characteristic: sample_attribution
characteristics.text: 102.100.100/401030
characteristics.characteristic: sample_id
characteristics.text: LakeMacquarie_Gwandalan3
characteristics.characteristic: sample_site_location_description
characteristics.text: Alessandra Suzzi
characteristics.characteristic: sample_submitter
characteristics.text: Sediment
characteristics.characteristic: sample_type
characteristics.text: http://hdl.handle.net/102.100.100/401030
characteristics.characteristic: source_mat_id
characteristics.text: frozen (-80°)
characteristics.characteristic: store_cond
characteristics.text: 22.31 degC
characteristics.characteristic: temp
characteristics.text: meth_Horiba U-50 water quality meter
characteristics.characteristic: temp_meth
characteristics.text: 102.100.100/401030
characteristics.characteristic: title
characteristics.text: https://data.bioplatforms.com//organization/australian-microbiome?q=sample_id:102.100.100/401030
characteristics.characteristic: url
characteristics.text: Seagrass bed/meadow
characteristics.characteristic: vegetation_type