characteristics.text: SAMN43497899
characteristics.characteristic: External Id
characteristics.text: USDA-ARS-USMARC
characteristics.characteristic: INSDC center name
characteristics.text: 2024-10-01T00:52:06.020Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-10-01T00:52:06.020Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: MIGS.ba
characteristics.characteristic: NCBI submission model
characteristics.text: MIGS.ba.6.0
characteristics.characteristic: NCBI submission package
characteristics.text: ERS23218731
characteristics.characteristic: SRA accession
characteristics.text: 2024-08-15
characteristics.characteristic: collection_date
characteristics.text: chemically defined minimal medium aerobic culture of NONSMOOTH colony Sample ID 209469
characteristics.characteristic: collection_method
characteristics.text: A glycerol stock of NONSMOOTH colonies (Sample ID 66058, a child of Sample ID 184, BioSample SAMN03275447 ) was plated on blood agar base + hemin (BABH) medium and grown overnight at 37C under 5% CO2 to generate NONSMOOTH colonies. A heavy streak of these NONSMOOTH colonies was struck out again on BABH yielding consistently NONSMOOTH colonies on BABH. A single colony (source material Sample ID 209469) on the BABH plate was selected for aerobic culture in chemically defined minimal medium (CDMM) in a 50 ml conical with 15 mL CDMM for 21 hours; 191 rpm 37C. Caps lightly cracked open. DNA was extracted from this culture for PacBio HiFi sequencing.
characteristics.characteristic: description
characteristics.text: NONSMOOTH phenotype
characteristics.characteristic: encoded_traits
characteristics.text: ENVO:00002123
characteristics.characteristic: env_broad_scale
characteristics.text: ENVO:00002124
characteristics.characteristic: env_local_scale
characteristics.text: MICRO:0000067
characteristics.characteristic: env_medium
characteristics.text: 2500000
characteristics.characteristic: estimated_size
characteristics.text: USA: Clay Center, NE
characteristics.characteristic: geo_loc_name
characteristics.text: Bos taurus
characteristics.characteristic: host
characteristics.text: Bovine Respiratory Disease Complex
characteristics.characteristic: host_disease
characteristics.text: 9913
characteristics.characteristic: host_taxid
characteristics.text: 5955799
characteristics.characteristic: isol_growth_condt
characteristics.text: Derived from an aerobic bioreactor culture in chemically defined minimal medium (CDMM) inoculated with a suspension of a single NONSMOOTH colony (Sample ID 209469) grown on blood agar base + hemin plate (BABH) medium. This BABH colony was isolated from a glycerol stock of NONSMOOTH colonies with Sample ID 66058, a child of Sample ID 184, BioSample SAMN03275447.
characteristics.characteristic: isolation_source
characteristics.text: NA
characteristics.characteristic: lat_lon
characteristics.text: 1
characteristics.characteristic: num_replicons
characteristics.text: Mannheimia haemolytica
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_75985
characteristics.characteristic: organism
characteristics.text: Bos taurus
characteristics.characteristic: pathogenicity
characteristics.text: NA
characteristics.characteristic: ref_biomaterial
characteristics.text: facultative
characteristics.characteristic: rel_to_oxygen
characteristics.text: 209469
characteristics.characteristic: source_material_id
characteristics.text: Mh184.20240815.PV.NSC
characteristics.characteristic: strain
characteristics.text: phase variant
characteristics.characteristic: subspecf_gen_lin
characteristics.text: Mannheimia haemolytica USDA-ARS-184 Phase Variant 20240815.NSC
characteristics.characteristic: title