characteristics.text: SAMN43682939
characteristics.characteristic: External Id
characteristics.text: National Institute of Standards and Technology
characteristics.characteristic: INSDC center name
characteristics.text: 2024-10-18T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-10-18T09:22:30.722Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS22636947
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Human
characteristics.characteristic: NCBI submission model
characteristics.text: Human.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS22636947
characteristics.characteristic: SRA accession
characteristics.text: 61
characteristics.characteristic: age
characteristics.text: Massachusetts General Hospital (MGH)
characteristics.characteristic: biomaterial_provider
characteristics.text: HG008-T
characteristics.characteristic: cell_line
characteristics.text: epithelial-like
characteristics.characteristic: cell_subtype
characteristics.text: pancreatic ductal adenocarcinoma
characteristics.characteristic: cell_type
characteristics.text: 2020-12-30
characteristics.characteristic: collection_date
characteristics.text: pancreatic ductal adenocarcinoma
characteristics.characteristic: disease
characteristics.text: tumor stage ypT2 N1, histological grade G2 (Moderately differentiated)
characteristics.characteristic: disease_stage
characteristics.text: USA
characteristics.characteristic: geo_loc_name
characteristics.text: yes
characteristics.characteristic: is_tumor
characteristics.text: GIAB HG008
characteristics.characteristic: isolate
characteristics.text: pancreatic ductal adenocarcinoma
characteristics.characteristic: isolation_source
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: The individual (known as GIAB HG008) was explicitly consented for tissue collection and public genomic data sharing following a Massachusetts General Hospital (MGH) IRB-approved protocol. The Liss laboratory at MGH established a PDAC tumor cell line (HG008-T) from the resected PDAC tumor. PDAC tumors are stromal-rich, with cancer cells often only composing 10% of the tumor. The cell line was developed starting with approximately 50 mg of tumor tissue being enzymatically dissociated to culture tumor cells. While PDAC cell cultures often contain substantial fibroblasts, all cells appeared to be tumor cells even at passage 2 for this cell line. Cells were cultured for the first 20 passages using "Rich" media composed of DMEM/F12 with 20% Fetal Bovine Serum (FBS) Nicotinamide (0.01 M), cholera toxin (8.4 ng/ml), Epidermal Growth Factor (EGF; 10 ng/ml), Hepatocyte Growth Factor (HGF: 25 ng/ml), human recombinant insulin (10 ug/ml), human recombinant transferrin (5.5 ug/ml), and selenious acid (6.7 ng/ml). Morphologically pure cancer cell cultures, free of tumor-associated fibroblasts, were considered cell lines after growing for at least 20 passages. Passages 21-24 were grown in the same media as prior passages however without EGF. From passage 25 on, the established cell line was then cultured in DMEM/F12 with 10% FBS. All media contained 1% antibiotic/antimycotic.
characteristics.characteristic: passage_history
characteristics.text: Cells were enzymatically removed from the culture surface using 0.25% trypsin and split by diluting no more than 1:3 for the first 20 passages. Passages 21 - 38 were generally split 1:3, with some split 1:4.
characteristics.characteristic: passage_method
characteristics.text: Liss_2022p38
characteristics.characteristic: passage_number
characteristics.text: cell culture
characteristics.characteristic: sample_type
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: EUR
characteristics.characteristic: super_population_code
characteristics.text: European
characteristics.characteristic: super_population_description
characteristics.text: Pancreatic Ductal Adenocarcinoma Cell Line (PDAC)
characteristics.characteristic: tissue
characteristics.text: Human sample from Homo sapiens
characteristics.characteristic: title
characteristics.text: neoadjuvant therapy, tumor regression grade 2 (Minimal response: Residual cancer outgrown by fibrosis)
characteristics.characteristic: treatment