characteristics.text: SAMD00536224
characteristics.characteristic: External Id
characteristics.text: Department of Pharmacology, School of Pharmaceutical Sciences, University of Shizuoka
characteristics.characteristic: INSDC center name
characteristics.text: 2024-09-21T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-09-21T09:02:19Z
characteristics.characteristic: INSDC last update
characteristics.text: DRS407131
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: DRS407131
characteristics.characteristic: SRA accession
characteristics.text: a 3-month-old mouse
characteristics.characteristic: age
characteristics.text: ATCC (American Type Culture Collection)
characteristics.characteristic: breed
characteristics.text: AML12
characteristics.characteristic: cell_line
characteristics.text: non-transformed murine hepatocyte line
characteristics.characteristic: cell_type
characteristics.text: 2022-05-26
characteristics.characteristic: collection_date
characteristics.text: Non-transformed murine hepatocyte line AML12 cells were obtained from the ATCC and maintained in DMEM/Ham's F-12 supplemented with 10% FBS, ITS-G Supplement, 40 ng/mL dexamethasone, and penicillin/streptomycin and incubated for overnight at 37 _C in a 95% air_5% CO2 atmosphere. The culture medium was replaced with a serum-free medium containing the same supplements to induce hepatocyte differentiation. RNA sequencing was performed after treating differentiated AML12 cells without PA for 48h. Total RNA was extracted using a Nucleo Spin RNA according to the instructions of the manufacturer.
characteristics.characteristic: description
characteristics.text: Normal
characteristics.characteristic: disease
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: CNT_2
characteristics.characteristic: sample_name
characteristics.text: cell culture
characteristics.characteristic: sample_type
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: liver
characteristics.characteristic: tissue
characteristics.text: AML12 treated with vehicle control_2
characteristics.characteristic: title