characteristics.text: SAMN44024817
characteristics.characteristic: External Id
characteristics.text: Department of Hematology, The Second Xiangya Hospital
characteristics.characteristic: INSDC center name
characteristics.text: 2024-11-20T00:50:19.543Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-11-20T00:50:19.543Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS22800028
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Generic
characteristics.characteristic: NCBI submission model
characteristics.text: Generic.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS22800028
characteristics.characteristic: SRA accession
characteristics.text: Human T cell
characteristics.characteristic: cell type
characteristics.text: missing
characteristics.characteristic: collection_date
characteristics.text: ITK deletion
characteristics.characteristic: genotype
characteristics.text: missing
characteristics.characteristic: geo_loc_name
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: PBMCs
characteristics.characteristic: source_name
characteristics.text: PBMCs
characteristics.characteristic: tissue
characteristics.text: CD19-CAR-T cells, ITK-KO, replicate3
characteristics.characteristic: title
characteristics.text: Cas9-ITK-sgRNA(sgRNA designed to delete ITK) RNP complex was electroporated into CD19-CAR-T cells, and then ITK-KO CD19-CAR-T cells were co-cultured with MEC1 cells at an Effector (E) to target (T) ratio of 2:1 for 48 hours before Fluorescence-activated cell sorting
characteristics.characteristic: treatment