characteristics.text: SAMN44052896
characteristics.characteristic: External Id
characteristics.text: CUCEI-Universidad de Guadalajara
characteristics.characteristic: INSDC center name
characteristics.text: 2024-10-03T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2024-10-07T11:40:37.933Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS22832349
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: MIMARKS.survey
characteristics.characteristic: NCBI submission model
characteristics.text: MIGS/MIMS/MIMARKS.wastewater
characteristics.characteristic: NCBI submission model
characteristics.text: MIMARKS.survey.wastewater.6.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS22832349
characteristics.characteristic: SRA accession
characteristics.text: 2022-03-28
characteristics.characteristic: collection_date
characteristics.text: An anaerobic digestion process was conducted in a laboratory scale Fixed-bed reactor (FBR) with a working volume of 8.4 L made of glass fiber and epoxy vinylester resin derakane momentum 411-350 (Ashland, Delaware, USA). This digester was implemented with two types of carrier materials: nylon pad (NP) and nylon with silicon carbide pad (NSCP). The NP, a 6x9 inches green pad (96, 3M Scotch-BriteTM) was composed of nylon fibers bonded with resin. In contrast, the NSCP, a grey pad (7448, 3M Scotch-BriteTM), also 6x9 inches in size, consisted of nylon fibers bonded with resin and embedded with mineral silicon carbide particles throughout the pad. Silicon carbide (SiC) is known for its semiconductor properties and exceptional corrosion resistance, rendering it suitable for various applications. These carrier materials were inserted into a support structure, which was placed within the FBR to facilitate biofilm formation. The digester was kept at 35 C and a pH of 7 by adding NaOH 3 M to operate under mesophilic and neutrophilic conditions during the AD process. The FBR was operated for 65 days, assessing the digester performance and biofilm formation on carrier materials during methane production. Batch feeding of tomato LF at 300 mL/min occurred with varied operating conditions in three stages: (I) inoculation and biofilm formation (20 days); (II) biofilm stimulation (22 days); and (III) biodegradability tests (23 days). The reactor was inoculated with anaerobic sludge (3.12 g-VS/L). Samples of suspended biomass (10 mL) were collected at the end of stages I and II, while adhered biomass to supporting materials (NP and NSCP) were obtained using 10 mL sterilized Phosphate Buffered Saline (PBS) buffer 1X solution to wash a pad volume of 4.0x1.5x0.9 cm at the end of stages III. Then, each sample was processed to metagenomic DNA extraction.
characteristics.characteristic: description
characteristics.text: Anaerobic reactor microbiome
characteristics.characteristic: env_broad_scale
characteristics.text: Laboratory-scale|fixed-bed|reactor
characteristics.characteristic: env_local_scale
characteristics.text: Tomate liquide fraction
characteristics.characteristic: env_medium
characteristics.text: Mexico: Orizaba
characteristics.characteristic: geo_loc_name
characteristics.text: Nylon pad and nylon with silicon carbide pad
characteristics.characteristic: isolation_source
characteristics.text: 18.51 N 97.06 W
characteristics.characteristic: lat_lon
characteristics.text: anaerobic digester metagenome
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_1263854
characteristics.characteristic: organism
characteristics.text: MIMARKS Survey related sample from anaerobic digester metagenome fed with tomato liquid fraction
characteristics.characteristic: title