characteristics.text: 2025-02-12
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Katowice, Poland
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS22949560
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-14769:Sample 5
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: domesticated barley
characteristics.characteristic: common name
characteristics.text: Sebastian
characteristics.characteristic: cultivar
characteristics.text: Protocols: The sample is a fragment of a barley leaf, collected from plants during the experiment. Leaves were excised using sterile scissors to avoid contamination and immediately frozen in liquid nitrogen to preserve RNA integrity. Samples were stored at -80°C until further processing.  To evaluate the impact of specific mutations in the HvSNAC1 gene on the stress response, both mutants and the parent variety 'Sebastian', which is wild type (WT) for mutants, underwent drought treatment using a protocol extensively detailed by Daszkowska-Golec et al. (2017). The drought treatment involved planting barley seeds in pots filled with a mixture of sandy loam, with sand (w/w; 7:2), and supplemented with a nutrient medium. Initially, barley seeds were germinated in Petri dishes containing water-soaked vermiculite and incubated in darkness at 4°C for two days. Subsequently, the germinated seedlings were transferred to a greenhouse for an additional two days before being transplanted into the soil, with 15 seedlings per pot. One pot was one biological replicate, and three biological replicates were tested for each genotype and condition. Soil moisture levels were monitored daily throughout the experiment using Time-domain reflectometer (TDR) EasyTest equipment provided by the Institute of Agrophysics, Polish Academy of Sciences, Poland.  The drought assay comprised of two main phases:  1. Control Growth (CG): Seedlings were grown under optimal conditions with soil moisture at 12% vwc for ten days after transplantation. 2. Drought Stress (DS): Seedlings were subjected to severe drought stress, first soil moisture was gradually reduced from 12 to 3% vwc over four days for adaptation to water deficit (AWD), then soil moisture was maintained at 3-1.5% vwc for ten days.  The entire drought test spanned 24 days. During the CG and AWD, seedlings were cultivated in a greenhouse at 20˚C/18˚C with a 16/8 hour photoperiod and 400 μEm-2s-1 light intensity from high pressure sodium lamps. After AWD, seedlings were transferred to a growth chamber with a temperature regime of 25˚C/20˚C, maintaining the same photoperiod and light intensity as in the greenhouse.   The RNAqueous™ Total RNA Isolation Kit (ThermoFisher Scientific) was used per the manufacturer protocol, but instead of a lysis buffer, a TriPure Isolation Reagent (Roche Life Science) was applied. Stranded cDNA library preparation (poly-A selection) was used.
characteristics.characteristic: description
characteristics.text: control
characteristics.characteristic: environmental stress
characteristics.text: Sebastian parent variety
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Hordeum vulgare subsp. vulgare
characteristics.characteristic: organism
characteristics.text: Hordeum vulgare subsp. vulgare
characteristics.characteristic: organism
characteristics.text: leaf
characteristics.characteristic: organism part
characteristics.text: Hordeum vulgare subsp. vulgare
characteristics.characteristic: scientific_name
characteristics.text: Sample 5
characteristics.characteristic: title