2025-02-13
ENA first public
ERC000013
ENA-CHECKLIST
HCMR
INSDC center name
public
INSDC status
ERS22989238
SRA accession
t3p2r2_1st_SB10
Submitter Id
2
biological_replicate
aquarium [ENVO_00002196]
broad-scale environmental context
2023-09-18
collection date
Microbiome of Chondrilla nucula South population individual B fragment 10 biorep2 North Aegean Climate Change (NACC) sample analyzed by 16SrRNA sequencing
description
organic material [ENVO_01000155]
environmental medium
7.8
experiment_pH
30
ºC
experiment_temperature
T703-T5F-t3p2r2-1st_S406_L001
filename_basename
Greece
geographic location (country and/or sea)
35.331944
DD
geographic location (latitude)
25.282222
DD
geographic location (longitude)
Chondrilla nucula
host scientific name
SB10
host subject id
220712
host taxid
Dual-indexing two-step PCR with Truseq adapters and diversity spacers in the primers
library construction method
508796
library reads sequenced
saline water aquarium [ENVO_00002197]
local environmental context
ZymoResearch QuickDNA Microprep kit
nucleic acid extraction
commensal
observed biotic relationship
sponge metagenome
organism
MACCIMO: Multi-level Approaches to assess Climate Change Impact to Marine Organisms
project name
moved to aquarium for a 3-month acclimatization, where temperature increased from 16°C to 24°C (rate:0.08°C/day), then subjected to an increase in temperature to 30°C (rate:0.06°C/day) and a decrease in pH from 8 to 7.8  (rate: 0.03 units/week) for 3 months
sample material processing
sponge metagenome
scientific_name
Illumina (MiSeq)
seq_meth
16S rRNA
target gene
V3-V4
target subfragment
South population individual B fragment 10 biorep2 NACC
title