2025-02-13
ENA first public
ERC000011
ENA-CHECKLIST
Wellcome Sanger Institute
INSDC center name
public
INSDC status
ERS23277159
SRA accession
E-MTAB-9536_2:FCA_gut9456322
Submitter Id
11.1
week
age
ArrayExpress
broker name
not collected
collection date
human
common name
Protocols: Human fetal tissues were obtained from the MRC/Wellcome Trust-funded Human Developmental Biology Resource(HDBR, http://www.hdbr.org) with appropriate maternal written consent and approval from the Newcastle and North Tyneside NHS Health Authority Joint Ethics Committee (08/H0906/21+5). Adult human tissue was obtained from deceased transplant organ donors after ethical approval (reference 15/EE/0152, East of England Cambridge South Research Ethics Committee) and informed consent from the donor family. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol.  Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. cDNA library preparation was were carried out according to the manufacturer’s protocol.
description
embryo
developmental stage
normal
disease
not collected
geographic location (country and/or sea)
BRC2258
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
Large intestine
organism part
Homo sapiens
scientific_name
FCA_gut9456322
title