2025-02-12
ENA first public
ERC000025
ENA-CHECKLIST
UNIVERSITY OF SZEGED
INSDC center name
public
INSDC status
ERS23137323
SRA accession
BACMIX_PRMT_GM1_GMS_MONT_20240807_bc22
Submitter Id
Top strand 5'-TTTTTTTTCCTGTACTTCGTTCAGTTACGTATTGCT-3'; Bottom strand 5'-ACGTAACTGAACGAAGTACAGG-3'
adapters
Csongrad county
broad-scale environmental context
2024-08-04
collection date
ZymoBIOMICS Gut Microbiome Standard isolated by ZymoBIOMICS 96 MagBead DNA Kit
description
ZymoBIOMICS Gut Microbiome Standard
environmental medium
Hungary
geographic location (country and/or sea)
46.248755
DD
geographic location (latitude)
20.145766
DD
geographic location (longitude)
16S barcoding (SQK-16S024)/ Native Barcoding Kit 94 V14 (SQK-LSK114.96)
library construction method
ligation based
library screening strategy
Szeged
local environmental context
ACCACTGCCATGTATCAAAGTACG/CGTACTTTGATACATGGCAGTGGT
multiplex identifiers
LongAmp HotStart Taq 2X Master Mix
nucleic acid amplification
ZymoBIOMICS 96 MagBead DNA Kit
nucleic acid extraction
mixed culture metagenome
organism
Initial denaturation 1 min 95 �C (1 cycle); Denaturation 20 secs 95 �C (25 cycles); Annealing 30 secs 55 �C (25 cycles); Extension 2 mins 65 �C (25 cycles); Final extension 5 mins 65 �C (1 cycle)
pcr conditions
27F 5'-(AGRGTTYGATYMTGGCTCAG); 1492R 5'-(CGGYTACCTTGTTACGACTT)
pcr primers
Evaluating Primer Bias in Full-Length 16S Sequencing of Synthetic and Host Fecal Samples
project name
mixed culture metagenome
scientific_name
software
sequence quality check
16S rRNA
target gene
V1-V9
target subfragment
PRMT_GM1_GMS_MONT_20240807_bc22
title