2025-02-12
ENA first public
ERC000013
ENA-CHECKLIST
UNIVERSITY OF SZEGED
INSDC center name
public
INSDC status
ERS21409625
SRA accession
Canfam_SRTP_A_M2_210704_ZHMW
Submitter Id
5�-TTTTTTTTCCTGTACTTCGTTCAGTTACGTATTGCT-3�
adapters
breeder's line
biological status
Danube Basin
broad-scale environmental context
2021-07-04
collection date
dog
common name
Dog stool sample of Sampo
description
no
environmental medium
Hungary
geographic location (country and/or sea)
47.31955
DD
geographic location (latitude)
19.324268
DD
geographic location (longitude)
D�ny
geographic location (region and locality)
NA
gravidity
8
years
host age
stool
host body product
Hungarian herding terrier: Pumi
host common name
Gemon adult lamb Rice/Gemon mini starter salmon 3:1
host diet
No relationship, same living environment
host family relationship
adult
host life stage
Canis lupus familiaris
host scientific name
male
host sex
SRTP_A_M2
host subject id
cross-breeding of Hungarian Puli with French and German herding dogs originated from 17. century
host subspecific genetic lineage
9615
host taxid
Rapid sequencing amplicons - 16S barcoding (SQK-RAB204)
library construction method
AMPLICON
library screening strategy
1500
library size
Garden
local environmental context
AAGAAAGTTGTCGGTGTCTTTGTG
multiplex identifiers
LongAmp Taq 2X Master Mix
nucleic acid amplification
Zymo Quick-DNA HMW MagBead Kit
nucleic acid extraction
Canis lupus familiaris
organism
Initial denaturation 1 min 95 °C (1 cycle); Denaturation 20 secs 95 °C (25 cycles); Annealing 30 secs 55 °C (25 cycles); Extension 2 mins 65 °C (25 cycles); Final extension 5 mins 65 °C (1 cycle)
pcr conditions
16SFw 5' - ATCGCCTACCGTGAC - barcode - AGAGTTTGATCMTGGCTCAG - 3'; 16S Rev 5' - ATCGCCTACCGTGAC - barcode - CGGTTACCTTGTTACGACTT - 3'
pcr primers
Metagenomic sequencing for longitudinal analysis of dog gut microbiome
project name
Eppendorf_CryoCube_F740h
sample storage location
-80
°C
sample storage temperature
0,1
g
sample volume or weight for DNA extraction
Canis lupus familiaris
scientific_name
16S rRNA
target gene
V1-V9
target subfragment
SRTP_A_M2_210704_ZHMW
title