SAMN44317475
External Id
USDA, ARS, USMARC
INSDC center name
2024-10-16T00:00:00Z
INSDC first public
2024-10-17T16:05:27.820Z
INSDC last update
SRS22938251
INSDC secondary accession
live
INSDC status
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
SRS22938251
SRA accession
5 years
age
USMARC
biomaterial_provider
1989
birth_date
USA: Nebraska
birth_location
Gelbvieh x Simental
breed
Gelvieh x Simmental
breeding_history
Semen
cell_type
1993
collection_date
Bovine viral diarrhea virus (BVDV) infection during pregnancy is a significant contributor to reproductive failures in cattle. The bovine receptor for BVDV (CD46) was previously edited with a six amino acid substitution (G82QVLAL to A82LPTFS) and shown to have significantly reduced BVDV susceptibility in a Gir heifer calf. Since a role for CD46 has been proposed in mammalian fertilization, our objective was to assess the edited heifer's fertilization rates, early embryonic development, and germline transmission conformation of the edit. Cumulus oocyte complexes were collected from the edited heifer and unedited females, fertilized with semen from an unedited bull and cultured until the blastocyst stage. Ultrasound examinations and serum progesterone concentration were also monitored to confirm estrous cyclicity in the CD46-edited heifer. Estrous cyclicity was normal with visualization of a corpus luteum and elevated progesterone concentrations. Fertilization rates and blastocyst development were not different in oocytes from edited and unedited controls. Genome sequence analysis of two blastocysts confirmed germline transmission of either edited allele from the heifer. Subsequently, the CD46-edited heifer was artificially inseminated with semen from an unedited Gir bull and fertility status was confirmed with a diagnosed conception at day 35 of gestation. Thus a six amino acid substitution in CD46 did not negatively affect fertilization of an edited oocyte or early embryonic development when fertilized with semen from an unedited bull. An edited bull is still needed to similarly evaluate reproductive function of sperm cells carrying this CD46 edit.
description
Adult
dev_stage
CD46 G82QVLAL/G82QVLAL
genotype
USA: Nebraska
geo_loc_name
Healthy
health_state
3
isolate
Bos taurus
http://purl.obolibrary.org/obo/NCBITaxon_9913
organism
male
sex
Liquid N2, 30 years
store_cond
19896800
strain
19896800
stud_book_number
Semen
tissue
Bovine IVF blastocyst 1 produced from Ginger's CD46-edited ALPTFS oocytes fertilized with Toro semen
title