2025-02-12
ENA first public
ERC000013
ENA-CHECKLIST
UNIVERSITY OF SZEGED
INSDC center name
public
INSDC status
ERS23179870
SRA accession
Canfam_PRMT_CP2_canis_PB
Submitter Id
Csongrad-Csanad county
broad-scale environmental context
2023-03-25
collection date
dog
common name
Dog stool sample
description
no
environmental medium
Hungary
geographic location (country and/or sea)
46.248755
DD
geographic location (latitude)
20.145766
DD
geographic location (longitude)
2.8
years
host age
female
host sex
16S barcoding (SQK-16S024)/ Native Barcoding Kit 94 V14 (SQK-LSK114.96)
library construction method
Szeged
local environmental context
CCAGTAGAAGTCCGACAACGTCAT/ATGACGTTGTCGGACTTCTACTGG
multiplex identifiers
ZymoBIOMICS 96 MagBead DNA Kit
nucleic acid extraction
Canis lupus familiaris
organism
27F 5'-(AGRGTTYGATYMTGGCTCAG); 1492R 5'-(RGYTACCTTGTTACGACTT)
pcr primers
Evaluating Primer Bias in Full-Length 16S Sequencing of Synthetic and Host Fecal Samples
project name
501
days
sample storage duration
-80
°C
sample storage temperature
Canis lupus familiaris
scientific_name
software
sequence quality check
Oxford Nanopore
sequencing method
16S rRNA
target gene
V1-V9
target subfragment
11
ºC
temperature
PRMT_CP2_canis_PB_20240807_bc32
title