SAMN44281841
External Id
Wisconsin State Laboratory of Hygiene
INSDC center name
2024-10-14T00:00:00Z
INSDC first public
2024-10-14T17:23:23.413Z
INSDC last update
SRS22902873
INSDC secondary accession
live
INSDC status
SARS-CoV-2: wastewater surveillance
NCBI submission model
SARS-CoV-2.wwsurv.1.0
NCBI submission package
SRS22902873
SRA accession
2024-09-30
collection_date
ceres nanotrap
concentration_method
bovine coronavirus
extraction_control
promega ht tna kit
extraction_method
USA: Wisconsin
geo_loc_name
wastewater
isolation_source
wastewater metagenome
http://purl.obolibrary.org/obo/NCBITaxon_527639
organism
Wisconsin SARS-CoV-2 Wastewater Monitoring Network
project_name
public health surveillance community-level
purpose_of_ww_sampling
Identification of the diversity of mutations across the SARS-CoV-2 metagenome
purpose_of_ww_sequencing
Wisconsin State Laboratory of Hygiene
sequenced_by
SARS-CoV-2: wastewater surveillance sample from wastewater metagenome
title
pepper mild mottle virus
ww_endog_control_1
66464000
ww_endog_control_1_conc
IDT PMMoV primer-probe set described in PMID 24056678 measured using dPCR QIAcuity instrument 96W plates (Qiagen).
ww_endog_control_1_protocol
copies/L
ww_endog_control_1_units
5133018.4008
ww_flow
7.3
ww_ph
<100000
ww_population
population of jurisdiction encompassing the wastewater service area
ww_population_source
not applicable
ww_primary_sludge_retention_time
Internal control spiked in samples at receipt. Samples were concentrated using Nanotrap Microbiome A beads combined with the enhancement reagent 2 (Ceres) on KingFisher Apex and extracted using Maxwell HT viral TNA kit (Promega) on KingFisher Flex.
ww_processing_protocol
24
ww_sample_duration
raw wastewater
ww_sample_matrix
wastewater treatment plant
ww_sample_site
composite
ww_sample_type
WI
ww_surv_jurisdiction
760211001
ww_surv_system_sample_id
SARS-CoV-2
ww_surv_target_1
153600
ww_surv_target_1_conc
copies/L
ww_surv_target_1_conc_unit
N gene (N1)
ww_surv_target_1_gene
yes
ww_surv_target_1_known_present
IDT SARS-CoV-2 Wastewater Surveillance CDC Assay For QIAcuity dPCR 24W (Qiagen)
ww_surv_target_1_protocol
SARS-CoV-2
ww_surv_target_2
205120
ww_surv_target_2_conc
copies/L
ww_surv_target_2_conc_unit
N gene (N2)
ww_surv_target_2_gene
yes
ww_surv_target_2_known_present
IDT SARS-CoV-2 Wastewater Surveillance CDC Assay For QIAcuity dPCR 24W (Qiagen)
ww_surv_target_2_protocol
8
ww_temperature