2025-02-12
ENA first public
ERC000013
ENA-CHECKLIST
UNIVERSITY OF SZEGED
INSDC center name
public
INSDC status
ERS21409662
SRA accession
Canfam_SRTP_A_M3_210213_ZMB
Submitter Id
5�-TTTTTTTTCCTGTACTTCGTTCAGTTACGTATTGCT-3�
adapters
breeder's line
biological status
Danube Basin
broad-scale environmental context
2021-02-13
collection date
dog
common name
Dog stool sample of Matyi
description
no
environmental medium
Hungary
geographic location (country and/or sea)
47.31955
DD
geographic location (latitude)
19.324268
DD
geographic location (longitude)
D�ny
geographic location (region and locality)
NA
gravidity
6
years
host age
stool
host body product
Hungarian herding terrier: Pumi
host common name
Monge Gemon Lamb-Rice
host diet
No relationship, same living environment
host family relationship
adult
host life stage
Canis lupus familiaris
host scientific name
male
host sex
SRTP_A_M3
host subject id
cross-breeding of Hungarian Puli with French and German herding dogs originated from 17. century
host subspecific genetic lineage
9615
host taxid
Rapid sequencing amplicons - 16S barcoding (SQK-16S024)
library construction method
AMPLICON
library screening strategy
1500
library size
Garden
local environmental context
CTTACTACCCAGTGAACCTCCTCG
multiplex identifiers
LongAmp Taq 2X Master Mix
nucleic acid amplification
ZymoBiomics 96 MagBead DNA Kit
nucleic acid extraction
Canis lupus familiaris
organism
Initial denaturation 1 min 95 °C (1 cycle); Denaturation 20 secs 95 °C (25 cycles); Annealing 30 secs 55 °C (25 cycles); Extension 2 mins 65 °C (25 cycles); Final extension 5 mins 65 °C (1 cycle)
pcr conditions
16SFw 5' - ATCGCCTACCGTGAC - barcode - AGAGTTTGATCMTGGCTCAG - 3'; 16S Rev 5' - ATCGCCTACCGTGAC - barcode - CGGTTACCTTGTTACGACTT - 3'
pcr primers
Metagenomic sequencing for longitudinal analysis of dog gut microbiome
project name
Eppendorf_CryoCube_F740h
sample storage location
-80
°C
sample storage temperature
0,1
g
sample volume or weight for DNA extraction
Canis lupus familiaris
scientific_name
16S rRNA
target gene
V1-V9
target subfragment
SRTP_A_M3_210213_ZMB
title