2025-02-14
ENA first public
ERC000011
ENA-CHECKLIST
Fondazione IRCCS Istituto Neurologico Carlo Besta
INSDC center name
public
INSDC status
ERS22903947
SRA accession
E-MTAB-14751:SR27
Submitter Id
ArrayExpress
broker name
SB28
cell_line
not collected
collection date
house mouse
common name
Protocols: After sacrificing the mice, brains were excised and tumors were selectively isolated. Tumor fractions were conserved in RNAlater for one week. Total RNA was extracted using TRIzol Reagent (Life Technologies, Thermo Fisher, cat n. 15596026) and RNeasy Mini Kit and RNase-Free DNase Set (Qiagen, cat n. 74104 and 79254). The concentration of RNA was quantified using a Qubit Fluorometer (Life Technologies, Carlsbad, CA) and RNA integrity was assayed using a TapeStation. Sequencing libraries were prepared with RNA with PolyA selection and ERCC spike-in with standard ERCC kit (ERCC RNA Spike-In Mix,Thermo Fisher 4456740).
description
glioblastoma
disease
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
Mus musculus
scientific_name
SR27
title