2025-02-14
ENA first public
ERC000011
ENA-CHECKLIST
Institute for Immunology and Hematology
INSDC center name
public
INSDC status
ERS23018921
SRA accession
E-MTAB-14801:FRC2-IR-RNA–WTIR
Submitter Id
10
week
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: Lymph node derived Fibroblast Reticular Cells (FRCs) were extracted from 10 week old male C57Bl6 mice either WT or TNCKO as previously described (Spenle et al., 2020, Cancer Immun Res, doi: 10.1158/2326-6066.CIR-20-0074). FRCs were plated for maximum 24 hours before 2 Gy irradiation, and then continued to culture for another 48 hours before collection. Total RNA was extracted using TriReagent (Life Technologies AM9738) Sequencing libraries were prepared using the Smarter Stranded Total RNA-Seq kit v3 Pico Input Mammalian following the manufacturer’s instructions (Takara Bio, San Jose, CA, USA)
description
adult
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
2
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
fibroblast reticular cell
organism part
Mus musculus
scientific_name
male
sex
ionizing irradiation
stimulus
C57Bl6
strain
FRC2-IR-RNA–WTIR
title