SAMN44752121
External Id
Air Force Research Laboratory
INSDC center name
2024-11-14T00:00:00Z
INSDC first public
2024-12-05T13:30:10.833Z
INSDC last update
SRS23241790
INSDC secondary accession
live
INSDC status
MIGS.eu
NCBI submission model
MIGS/MIMS/MIMARKS.built
NCBI submission model
MIGS.eu.built.6.0
NCBI submission package
SRS23241790
SRA accession
not collected
abs_air_humidity
not collected
air_temp
not applicable
build_occup_type
not applicable
building_setting
not collected
carb_dioxide
2020
collection_date
Keywords: GSC:MIxS;MIGS:6.0
description
not applicable
env_broad_scale
not applicable
env_local_scale
polymer coated surface
env_medium
not applicable
filter_type
USA: Florida
geo_loc_name
not applicable
heat_cool_type
not applicable
indoor_space
Cultures were recovered from glycerol freezer stocks by streaking onto potato dextrose agar (PDA). An isolated colony was re-suspended in tryptic soy broth (TSB) and grown overnight at 27C on a rotary wheel. A 1 mL aliquot of overnight culture was harvested and the pellet was used for DNA extractions. Genomic DNA was extracted using the Qiagen Power Microbiome kit according to the manufacturer's instructions and the DNA was quantified using the Qubit dsDNA assay (Life Technologies). Sequencing libraries were prepared using the Nextera DNA Flex Library preparation kit following the manufacturers instructions (Illumina, San Diego, CA) and sequenced using 150bp paired-end sequencing on an Illumina NextSeq 550.
isol_growth_condt
restricted access
lat_lon
not applicable
light_type
not applicable
occup_samp
not applicable
occupant_dens_samp
Aureobasidium sp. 76
http://purl.obolibrary.org/obo/NCBITaxon_3381320
organism
not collected
organism_count
not collected
rel_air_humidity
not applicable
space_typ_state
76
strain
MIGS Eukaryotic sample from Aureobasidium sp. 76
title
not applicable
typ_occupant_dens
not applicable
ventilation_type