SAMN44833514
External Id
Consiglio Nazionale delle Ricerche (CNR)
INSDC center name
2024-11-20T00:00:00Z
INSDC first public
2024-11-20T06:04:20.563Z
INSDC last update
SRS23262595
INSDC secondary accession
live
INSDC status
Plant
NCBI submission model
Plant.1.0
NCBI submission package
SRS23262595
SRA accession
31 days
age
2019-01-01
collection_date
Quadrato dAsti
cultivar
inoculated
description
Italy
geo_loc_name
Capsicum annuum
http://purl.obolibrary.org/obo/NCBITaxon_4072
organism
roots
tissue
Plant sample from Capsicum annuum
title
Seeds were washed in 5 ml of 0.1% Tween-20 for 20 min and surface sterilized in 5 ml of 2.5% NaOCl, rinsed 4-5 times in sterile distilled water (SDW) by gently shaking for 5 min each. The seeds were dried on autoclaved filter paper and then placed for germination on 1.5% water agar (WA) in dark at 26C. Assays were carried out in 750 ml glass bottles with 200 ml vermiculite and 150 ml SDW. The bottles were plugged with cotton and autoclaved twice for 20 min at 121C, prior receiving a 10-days germinated plantlet each. Subsequently, 6 agar plugs (0,5 cm2) from one week-old corn meal agar cultures of Pochonia chlamydosporia were added for the colonization treatment in the bottles, and mixed up before transplanting. All plants were maintained up to 21 days at 26C with 16 hrs illumination in a growth chamber (Sanyo Electric MLR-351, Osaka, Japan).
treatment