SAMN44672025
External Id
Liubov Skorodumova
INSDC center name
2024-11-11T00:00:00Z
INSDC first public
2024-11-11T07:01:07.510Z
INSDC last update
SRS23176063
INSDC secondary accession
live
INSDC status
Human
NCBI submission model
Human.1.0
NCBI submission package
SRS23176063
SRA accession
34
age
Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Malaya Pirogovskaya street 1a, Moscow, 119435 Russia
biomaterial_provider
yes
bowman's_membrane_and_corneal_stroma_included
2020-08-20
collection_date
no
corneal_epithelium_included
peripheral
corneal_part
A patient with keratoconus underwent deep anterior lamellar keratoplasty. The corneal sample included the following corneal layers: Bowman's membrane, and stroma.The sample was a 1.5 mm wide crescent shaped piece of the peripheral part of the cornea. Immediately after excision, the sample was preserved in RNAlater solution (ThermoFisher Scientific, Waltham, Massachusetts, USA). The sample was homogenized using a TissueLyser (Qiagen, Germany) with 4.75 mm diameter stainless steel beads for 30 seconds at maximum speed. Total RNA was isolated using the RNeasy Mini Kit (Qiagen, Germany) according to the manufacturer's protocol.
description
keratoconus
disease
4 (Amsler-Krumeich)
disease_stage
Russia: Moscow
geo_loc_name
KC_22
isolate
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
tissue sample
sample_type
male
sex
cornea
tissue
RNA from keratoconus corneal sample containing Bowman's membrane and stroma
title