SAMN45129488
External Id
U.S. Food and Drug Administration
INSDC center name
2024-12-02T00:00:00Z
INSDC first public
2024-12-05T12:03:41.117Z
INSDC last update
SRS23449486
INSDC secondary accession
live
INSDC status
MIGS/MIMS/MIMARKS.food-farm_env
NCBI submission model
MIMS.me
NCBI submission model
MIMS.me.food-farm_env.6.0
NCBI submission package
SRS23449486
SRA accession
farm [ENVO_00000078]
adjacent_environment
none
biotic_regm
none
chem_administration
2020-02-21
collection_date
sterile scoop
collection_method
surface
depth
Metagenome from enriched untreated dairy manure taken 1 day after amendment on agricultural soil. Sample replicate B.
description
FDA BAM Chapter 4A|https://www.fda.gov/food/laboratory-methods-food/bam-chapter-4a-diarrheagenic-escherichia-coli
enrichment_protocol
anthropogenic terrestrial biome [ENVO_01000219]
env_broad_scale
farm [ENVO_00000078]
env_local_scale
animal manure [ENVO_00003031]
env_medium
not applicable
food_product_type
USA:Ohio
geo_loc_name
70%
humidity
not applicable
ifsac_category
untreated dairy manure amendment
isolation_source
40.7589 N 81.9535 W
lat_lon
feces metagenome
http://purl.obolibrary.org/obo/NCBITaxon_1861841
organism
research
purpose_of_sampling
from the crop-growing fields
rel_location
US FDA
repository
sterile scoop
samp_collect_device
natural environment specimen [GENEPIO_0001250]
samp_mat_type
manure sample was a composite of 5 subsamples taken from the field
samp_pooling
2L
samp_size
natural environment specimen [GENEPIO_0001250]
samp_source_mat_cat
24-48 hours before enrichment
samp_store_dur
4 degrees during transport; -20 until processing
samp_store_temp
50g used for enrichment; 2ml enrichment used for DNA extraction
samp_vol_we_dna_ext
winter
season
79.16%
season_humidity
0.24cm
season_precpt
2.07C
season_temp
0.6C
soil_temp
silt loam
soil_texture_class
Hydrometer Method. ASTM D422, 2674
soil_texture_meth
2020-02-21
timepoint
E-M03b
title
150.6 degrees
wind_direction
2.0 kmph
wind_speed