SAMN45085216
External Id
Northwestern University
INSDC center name
2024-11-27T00:00:00Z
INSDC first public
2024-11-27T18:35:20.977Z
INSDC last update
SRS23384823
INSDC secondary accession
live
INSDC status
Human
NCBI submission model
Human.1.0
NCBI submission package
SRS23384823
SRA accession
n/a
age
Dr. Paul D. Bieniasz
biomaterial_provider
HT1080
cell_line
Epithelial
cell_type
n/a
collection_date
HT1080-CPSF6-mNGreen cells were infected with VSV-G pseudotyped wild type or N74D capsid mutant HIV-1-NL4-3 at a multiplicity of infection (MOI) of 10 by spinoculation or mock-infected. RNA was extracted from each treatment group at 6 hours post-infection, and RNA samples were used for next-generation sequencing. Libraries were prepared using a NEBNext Ultra II kit with poly(A) selection, and samples were sequenced using a NovaSeq X Plus with 150 bp paired-end reads.
description
Fibrosarcoma
disease
n/a
geo_loc_name
HT1080-CPSF6-mNGreen cells
isolate
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
Cell culture
sample_type
male
sex
Epithelial cells derived from connective tissue from a patient with Fibrosarcoma
tissue
Human sample from Homo sapiens
title
Infected with VSV-G Pseudotyped HIV-1 NL4-3 with N74D capsid mutation, Technical Replicate 3
treatment