SAMN45129542
External Id
U.S. Food and Drug Administration
INSDC center name
2024-12-02T00:00:00Z
INSDC first public
2024-12-05T12:03:31.357Z
INSDC last update
SRS23449419
INSDC secondary accession
live
INSDC status
MIGS/MIMS/MIMARKS.food-farm_env
NCBI submission model
MIMS.me
NCBI submission model
MIMS.me.food-farm_env.6.0
NCBI submission package
SRS23449419
SRA accession
farm [ENVO_00000078]
adjacent_environment
none
biotic_regm
none
chem_administration
2021-11-09
collection_date
sterile scoop
collection_method
surface
depth
Metagenome of enriched agricultural soil that does not use BSAAOs, taken 282 day after amendment in comparison field. Sample replicate A.
description
FDA BAM Chapter 4A|https://www.fda.gov/food/laboratory-methods-food/bam-chapter-4a-diarrheagenic-escherichia-coli
enrichment_protocol
anthropogenic terrestrial biome [ENVO_01000219]
env_broad_scale
farm [ENVO_00000078]
env_local_scale
agricultural soil [ENVO_00002259]
env_medium
not applicable
food_product_type
USA:Ohio
geo_loc_name
72%
humidity
not applicable
ifsac_category
agricultural soil
isolation_source
40.7589 N 81.9535 W
lat_lon
soil metagenome
http://purl.obolibrary.org/obo/NCBITaxon_410658
organism
tilling; 2021-03-26
perturbation
6.6
ph
1:1 in H20 McLean, E.O. 1982. Soil pH and lime requirement. p. 199-223. In A.L. Page et al. (ed.) Methods of soil analysis, part 2. Agronomy Monogr. 9, 2nd ed. ASA and SSSA, Madison, WI.
ph_meth
research
purpose_of_sampling
from the crop-growing fields
rel_location
US FDA
repository
sterile scoop
samp_collect_device
natural environment specimen [GENEPIO_0001250]
samp_mat_type
soil sample was a composite of 5 subsamples taken from the field
samp_pooling
2L
samp_size
natural environment specimen [GENEPIO_0001250]
samp_source_mat_cat
24-48 hours before enrichment
samp_store_dur
4 degrees during transport; -20 until processing
samp_store_temp
50g used for enrichment; 2ml enrichment used for DNA extraction
samp_vol_we_dna_ext
fall
season
80.31%
season_humidity
0.22cm
season_precpt
9.02C
season_temp
6.6
soil_ph
9.3C
soil_temp
loam
soil_texture_class
Hydrometer Method. ASTM D422, 2820
soil_texture_meth
2021-11-09
timepoint
E-N33a
title
water extractable organic carbon Nelson, D.W., and L.E. Sommers. 1996. Total carbon, organic carbon and organic matter. P. 961-1010. In J.M. Bartels et al. (ed.) Methods of soil analysis: Part 3 Chemical methods. (3rd ed.) ASA and SSSA Book Series 5, Madison, WI.
tot_org_c_meth
.2
tot_org_carb
116.5 degrees
wind_direction
1.0 kmph
wind_speed