Tuck029_A_water_cytb
EESC_lab_processing_ID
SAMN45166770
External Id
USGS Eastern Ecological Science Center
INSDC center name
2024-12-04T00:00:00Z
INSDC first public
2024-12-06T10:21:24.633Z
INSDC last update
SRS23460489
INSDC secondary accession
live
INSDC status
MIGS/MIMS/MIMARKS.water
NCBI submission model
MIMARKS.survey
NCBI submission model
MIMARKS.survey.water.6.0
NCBI submission package
SRS23460489
SRA accession
2020-07
collection_date
Surface
depth
Keywords: GSC:MIxS;MIMARKS:6.0
description
SmithRootSelfpreserving
eDNA_filter_type
freshwater stream biome [ENVO:03605008]
env_broad_scale
freshwater stream [ENVO:03605007]
env_local_scale
freshwater [ENVO:00002011]
env_medium
5.0
filter_pore_micron_size
USA: Virginia, Tuckahoe Creek, tributary to James River
geo_loc_name
37.6765 N 77.6900 W
lat_lon
Smith Root filters were extracted using the Qiagen DNEasy kit with some modifications. Inhibitors were removed from extracted DNA using the Zymo OneStep PCR Cleanup kit.
nucl_acid_ext
metagenome
http://purl.obolibrary.org/obo/NCBITaxon_256318
organism
FWD(cytb-F):5_GCCTACGCYATYCTHCGMTCHATYCC, REV(cytb-R):5_GGGTGTTCNACNGGYATNCCNCCAATTCA
pcr_primers
Illumina MiSeq with v3 chemistry generating paired end 250 bp reads
seq_meth
mitochondrial cytochrome b fragment
target_gene
Tuck029_A_water_cytb
title