2025-02-21
ENA first public
ERC000011
ENA-CHECKLIST
weizmann insitute of science
INSDC center name
public
INSDC status
ERS22993097
SRA accession
E-MTAB-14787:Sample 7
Submitter Id
14
age
ArrayExpress
broker name
not collected
collection date
human
common name
Protocols: we obtained fresh blood from donors identified as MBD4 mutation carriers dna was extracted from peripheral blood, using qiagen dna easy kit, per manufacture protocol.  Sample concentrations are normalized to 2nM and loaded onto an Illumina NovaSeq X Plus flow cell at a concentration that yields at least 2.5 B clusters of passing filter data per lane. The loading concentration for WES libraries has been optimized to maximize both well occupancy and unique read output while limiting duplicates associated with patterned flow cell technology. Samples are sequenced using 100 bp paired- end sequencing reads according to Illumina protocols. The 10bp indexes are read during additional sequencing reads that automatically follow the completion of read 1. Data generated during sequencing runs are simultaneously transferred to the YCGA high-performance computing cluster. A positive control (prepared bacteriophage Phi X library) provided by Illumina is spiked into every lane at a concentration of 1% to monitor sequencing quality in real time.
description
adolescent
developmental stage
mbd4 heterozygous
genotype
not collected
geographic location (country and/or sea)
n_8
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
blood
organism part
Homo sapiens
scientific_name
male
sex
Sample 7
title