2025-02-21
ENA first public
ERC000011
ENA-CHECKLIST
Wellcome Sanger Institute
INSDC center name
public
INSDC status
ERS23715647
SRA accession
E-MTAB-9543_4:Human_colon_16S8000477
Submitter Id
65 to 70
year
age
ArrayExpress
broker name
CD45 negative
cell_type
not collected
collection date
human
common name
Protocols:  For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol.  Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. cDNA library preparation was were carried out according to the manufacturer’s protocol.
description
adult
developmental stage
not collected
geographic location (country and/or sea)
417C
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
descending colon
organism part
Homo sapiens
scientific_name
male
sex
Human_colon_16S8000477
title