2025-02-24
ENA first public
ERC000011
ENA-CHECKLIST
National Cancer Institute
INSDC center name
public
INSDC status
ERS23678729
SRA accession
E-MTAB-14870:SM_CW1
Submitter Id
ArrayExpress
broker name
SKOV3
cell_line
Cancer cell line
cell_type
not collected
collection date
human
common name
Protocols: Cells were cultured under standard conditions at 37°C in a humidified atmosphere with 5% CO₂. SKOV3 S1 and S2 cells were collected for RNA extraction. Cells were washed with PBS, trypsinized using Trypsin-EDTA (0.25%), and pelleted by centrifugation at 300 × g for 5 minutes. The cell pellets were immediately flash-frozen in liquid nitrogen and stored at -80°C until RNA extraction. Total RNA was extracted using the TRIzol reagent (Invitrogen™, cat. no. 15596018) in combination with the RNeasy Mini Kit (Qiagen, cat. no. 74104), following the manufacturer’s protocol. RNA concentration and purity were assessed using a NanoDrop spectrophotometer (Thermo Scientific™ NanoDrop™ 2000). The 260/280 absorbance ratio was used as an indicator of RNA purity, with values between 1.8 and 2.0 considered acceptable. RNA samples were stored at -80°C until further use. Bulk RNA barcoding and sequencing (BRB-seq), and pre-processing of the data (demultiplexing and alignment) was performed by Alithea Genomics, as described previously Alpern, D. et al. BRB-seq: ultra-affordable high-throughput transcriptomics enabled by bulk RNA barcoding and sequencing. Genome Biol. 20, 71 (2019).
description
adult
developmental stage
Ovarian serous cystadenocarcinoma
disease
N/A
genotype
not collected
geographic location (country and/or sea)
2D, 3D
growth condition
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
ovary
organism part
Homo sapiens
scientific_name
S1, S2
strain
SM_CW1
title