Cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS), 100 U/ml penicillin and 100 g/ml streptomycin (PEST) (all from GE Healthcare HyClone). Cultured under standard culturing conditions (37oC, 5% CO2). Sent for mycoplasma testing (Eurofin Genomics) and confirmed as mycoplasma free. Sent for short tandem repeat (STR) profiling (Eurofins Genomics) to confirm cell line origin.
Culture information
SAMN45007880
External Id
Lund University
INSDC center name
2025-02-21T00:00:00Z
INSDC first public
2025-02-21T04:50:02.177Z
INSDC last update
SRS23330473
INSDC secondary accession
live
INSDC status
Human
NCBI submission model
Human.1.0
NCBI submission package
SRS23330473
SRA accession
passage 12
age
Johan Staaf, Translational Cancer Research, Laboratory Medicine, Lund University, Scheelevagen 2, 22381 Lund, Sweden
biomaterial_provider
BT-549
cell_line
2020-11
collection_date
Breast cancer
disease
Sweden: Lund
geo_loc_name
Cancer cell line
isolate
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
female
sex
Breast
tissue
Human sample from Homo sapiens
title