2025-02-26
ENA first public
ERC000011
ENA-CHECKLIST
US Food and Drug Administration, Center for Biologics Evaluation and Research
INSDC center name
public
INSDC status
ERS19010919
SRA accession
E-MTAB-14011:Sample 12
Submitter Id
ArrayExpress
broker name
not collected
collection date
human
common name
1000
cells per milliliter
concentration
Protocols: Human blood or plasma samples from anonymous donors are spiked with pathogen stocks in the laboratory Nucleic acids from plasma spiked with viral pathogens were extracted with the QIAamp MinElute Virus Spin kit (Qiagen, Valencia, CA) and DNA from bacteria or protozoan parasite spiked whole blood specimens was extracted with the DNA Blood Mini kit (QIAamp, Qiagen,) according to the manufacturers’ protocols PCR Amplification Nucleic acid samples were added to a reverse transcription (RT) reaction. The products from the RT reaction were divided among four PCR reactions each containing one of the primer pools with internal positive controls and the primer-linker oligonucleotide. After several rounds of specific amplification, the primer-linker continues the amplification of the existing PCR products. The PCR products were combined and purified with a DNA clean-up system (Wizard SV Gel and PCR Clean-Up System, A9282, Promega, Madison, WI). PCR product samples were used to prepare a library with the Oxford Nanopore Amplicons-by-Ligation kit (SQK-LSK112) following the manufacturer’s instructions.
description
not collected
geographic location (country and/or sea)
Plasmodium vivax
infect
not applicable
isolate
Human Blood
matrix
Homo sapiens
organism
Homo sapiens
organism
blood
organism part
Homo sapiens
scientific_name
Sample 12
title