2025-02-27
ENA first public
ERC000011
ENA-CHECKLIST
UNIFR
INSDC center name
public
INSDC status
ERS23238980
SRA accession
E-MTAB-14824:GFP-Dam_int_Rep2
Submitter Id
ArrayExpress
broker name
not collected
collection date
Protocols: C. elegans strains were grown on a lawn of GM48 (-dam) E.coli bacteria on NGM plates at 20°C for at least two generations. After synchronization, around 1500 L1 larvae were seeded on three 100 mm plates. The larvae were grown till the young adult stage and were collected after washing 10-12 times with M9 media in aliquots of 30-50 µl. After the removal of excess liquid, samples (approximately 4500 worms per sample) were snap frozen and stored at -80°C until further use. The list of strains used in the study is mentioned in the supplementary files DNA extraction from frozen worm pellets was performed by using the DNeasy Blood and Tissue Kit (#69504; Qiagen, Valencia, CA).  500 ng of DNA was used for every sample for digestion with dpnI followed by ligation of the adaptors and PCR amplification to generate DNA amplicons of each sample. For the intestinal DamID 18-22 PCR cycles were performed (sup Figure 1B, representative replicate 1). DNA amplicons purifications were performed after every step from the PCR amplification onwards using magnetic beads and DNA quantification was done using QUBIT. Library preparation included end repair, barcoding and pooling of the libraries to be loaded on sequencer.
description
newly molted young adult hermaphrodite
developmental stage
heSi142[elt-2p::elg-13nls::Cre::tbb-2 3'UTR] X; ubsSi40[hsp-16.41::lox:mcherry::lox::degron::gfp::dam::unc-54 3'UTR; unc-119(+)] IV
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Caenorhabditis elegans
organism
Caenorhabditis elegans
organism
intestine
organism part
Caenorhabditis elegans
scientific_name
hermaphrodite
sex
DAG1043
strain
GFP-Dam_int_Rep2
title