2025-02-28
ENA first public
ERC000011
ENA-CHECKLIST
DKFZ Heidelberg
INSDC center name
public
INSDC status
ERS22966132
SRA accession
E-MTAB-12906_2:Exp2_Sample8_S4
Submitter Id
ArrayExpress
broker name
mixture (immune, endothelial, fibroblasts, cancer cells)
cell_type
not collected
collection date
house mouse
common name
Protocols: Digested xenograft, electroporated tumor and mice pancreas cells were stained with a total of 3 fluorescent cell surface antibodies and DAPI. Cells were sorted into four populations according to CD326+ (EPCAM+), EPCAM-/CD31+, EPCAM-/CD45+ and EPCAM-/CD31-/CD45- expression. Cells from each population were sorted in PBS+5%BSA and mixed in a 2:1:1:1 ratio. 30.000 cells of each sample were further processed for single cell analysis. The single cell analysis of full tumor and pancreas was carried out with 10X Genomics Chromium Single Cell Kit Version 3,. Suspensions were prepared as described above and diluted in PBS, 30.000 cells were used as input and added to 10x Chromium RT mix. For downstream cDNA synthesis (12-14 PCR cycles), library preparation, and sequencing, we followed the manufacturer’s instructions.  The single cell analysis of full tumor and pancreas was carried out with 10X Genomics Chromium Single Cell Kit Version 3,. Suspensions were prepared as described above and diluted in PBS, 30.000 cells were used as input and added to 10x Chromium RT mix. For downstream cDNA synthesis (12-14 PCR cycles), library preparation, and sequencing, we followed the manufacturer’s instructions.
description
adult
developmental stage
pancreatic ductal adenocarcinoma
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
Exp2_Sample8
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
pancreas
organism part
Mus musculus
scientific_name
male
sex
stimulus
NSG
strain
Exp2_Sample8_S4
title