2025-02-28
ENA first public
ERC000013
ENA-CHECKLIST
UNIVERSITY OF SZEGED
INSDC center name
public
INSDC status
ERS23237317
SRA accession
HoSa_PRMT_HO3_human_ONT_20240807_bc15
Submitter Id
Fejer
broad-scale environmental context
2022-02-11
collection date
human
common name
Human stool sample
description
no
environmental medium
Hungary
geographic location (country and/or sea)
47.1888
DD
geographic location (latitude)
18.4137
DD
geographic location (longitude)
53
years
host age
female
host sex
16S barcoding (SQK-16S024)/ Native Barcoding Kit 94 V14 (SQK-LSK114.96)
library construction method
Szekesfehervar
local environmental context
AGGTCTACCTCGCTAACACCACTG/CAGTGGTGTTAGCGAGGTAGACCT
multiplex identifiers
ZymoBIOMICS 96 MagBead DNA Kit
nucleic acid extraction
Homo sapiens
organism
27F 5'-(AGAGTTTGATCCTGGCTCAG); 1492R 5'-(CGGTTACCTTGTTACGACTT)
pcr primers
Evaluating Primer Bias in Full-Length 16S Sequencing of Synthetic and Host Fecal Samples
project name
908
days
sample storage duration
-80
°C
sample storage temperature
Homo sapiens
scientific_name
software
sequence quality check
Oxford Nanopore
sequencing method
16S rRNA
target gene
V1-V9
target subfragment
5
ºC
temperature
PRMT_HO3_human_ONT_20240807_bc15
title