2025-02-28
ENA first public
ERC000011
ENA-CHECKLIST
Centro Nacional de Biotecnología (CNB-CSIC) Plant Molecular Genetics Department
INSDC center name
public
INSDC status
ERS20251584
SRA accession
E-MTAB-14181:D6_Na2
Submitter Id
ArrayExpress
broker name
not collected
collection date
potato
common name
Protocols: Roots were collected at ZT10 (time point 6h) and the following day at ZT4 (time point 24h). Three biological replicates were used and each biological replicate is composed of the roots of three plants. Tissues were flash-frozen in liquid nitrogen and subsequently ground with a mortar.  Plants were grown in hydroponic conditions (0.5MS, 0.025mM MES). Plants were grown in a growth chamber with a 16h light / 8h dark light cycle.  NaCl was added to the hydroponic tanks to a final concentration of 125mM  RNA was extracted with FavorPrep™ Plant Total RNA Mini Kit from FAVORGEN. DNA was degraded in the column with RNase-free DNase I (Roche).  RNA library preparation was conducted by Novogene Co., LTD (Cambridge, United Kingdom)
description
Desirée
genotype
not collected
geographic location (country and/or sea)
hydroponics
growth condition
not applicable
isolate
Solanum tuberosum
organism
Solanum tuberosum
organism
root structure
organism part
Solanum tuberosum
scientific_name
Salt (125mM)
stimulus
D6_Na2
title