2025-02-28
ENA first public
ERC000011
ENA-CHECKLIST
UNIVERSITYOFABERDEEN
INSDC center name
public
INSDC status
ERS23170167
SRA accession
E-MTAB-14816:IAA_1
Submitter Id
ArrayExpress
broker name
not collected
collection date
Protocols: Worms were washed off NGM plates using M9 and washed 3 times by centrifugation at 749g for 2 min at 4°C.  1 ml of Trizol (TRI Reagent, T9424, Sigma Aldrich) was added per 50 µl of worm pellet and samples frozen in liquid N2. Samples were thawed at 37°C and re-frozen four times. They were then vortexed for 30 seconds, left to rest for 30 seconds and the vortex/rest process repeated for a further three times. Phase separation by addition of chloroform and recovery of RNA was done as described by the manufacturer.  The aqueous fraction was transferred to a low DNA-binding-tube and diluted 1:1 with 70% ethanol. RNA was purified using PureLink RNA Mini Kit (Thermo Fisher Scientific), using the on-column DNase-treatment option.  Directional library preparation, with rRNA removal was performed (Novogene UK Ltd).
description
L4 larva
developmental stage
not collected
geographic location (country and/or sea)
not applicable
isolate
Caenorhabditis elegans
organism
Caenorhabditis elegans
organism
Caenorhabditis elegans
scientific_name
hermaphrodite
sex
PE1176
strain
IAA_1
title