2025-03-01
ENA first public
ERC000011
ENA-CHECKLIST
Department of Clinical Science, University of Bergen, Norway
INSDC center name
public
INSDC status
ERS22971809
SRA accession
E-MTAB-14774:AireC313X_3_Thymus_TCR_Lane4
Submitter Id
64
day
age
ArrayExpress
broker name
immune cell
cell_type
not collected
collection date
house mouse
common name
Protocols: Thymi and lymph nodes were squashed and filtered through 70 μm MACS SmartStrainers (Miltenyi Biotech, cat. 130-110-916) and washed with 0.5% fetal bovine serum (FBS; Gibco, cat. 10082147) in phosphate-buffered saline (PBS).  Bone marrow immune cells were isolated by washing with with 0.5% fetal bovine serum (FBS; Gibco, cat. 10082147) in phosphate-buffered saline (PBS) Dead dells from all three tissues were subsequently removed with Dead cell removal kit (Miltenyi Biotech, cat. 130-090-101).  Ten thousand cells (counted in duplicate) from each thymus, lymph node and bore marrow sample were prepared with Chromium Next GEM Single Cell 5' Kit v2 (10x Genomics, cat. PN-1000286) and run on the Chromium Controller (10x Genomics). Gene expression libraries were prepared using the same single cell kits, V(D)J libraries were prepared with Chromium Single Cell Mouse TCR Amplification Kit (10x Genomics, cat. PN-1000254) and Chromium Single Cell Mouse BCR Amplification Kit (10x Genomics, cat. PN-1000255) and additional Library Construction Kits (10x Genomics, cat. PN-000352).
description
postnatal
developmental stage
AireC313X
genotype
not collected
geographic location (country and/or sea)
AireC313X_3
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
thymus
organism part
Mus musculus
scientific_name
female
sex
C57Bl/6
strain
AireC313X_3_Thymus_TCR_Lane4
title