2025-03-01
ENA first public
ERC000011
ENA-CHECKLIST
University of Chicago
INSDC center name
public
INSDC status
ERS20600601
SRA accession
E-MTAB-14273:input_MP1_rep1_MP7812
Submitter Id
ArrayExpress
broker name
16HBE14o-
cell_line
epithelial cell of bronchus
cell_type
not collected
collection date
human
common name
Protocols: 16HBE14O- were purchased and maintained using standard protocols. mRNA was converted to cDNA using Superscript III Reverse Transcriptase. cDNA was treated with RNAse A (invitrogen #12091-021) and RNAse T1 (ThermoFisher #EN0541) for one hour and then cleaned with the Qiagen Minelute PCR purification kit. After transfection, cells were lysed using a 20g needle and RNA was extracted using Qiagen RNeasy mini kit.  Libraries were amplified with 10-15 PCR cycles using Q5 Hot Start High-Fidelity 2x Master Mix (NEB #M0494S) and pooled before clean up using Agencourt AMPure XP beads (0.6x + 1.2x double cleanup; Beckman Coulter; #A63882).
description
normal
disease
1
experiment id
not collected
geographic location (country and/or sea)
1
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
lung
organism part
input
sample type
Homo sapiens
scientific_name
male
sex
input_MP1_rep1_MP7812
title