2025-03-02
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA112716316
External Id
Institut fur Pharmakologie Medizinische Fakultat der Heinrich-Heine-Universitat Dusseldorf
INSDC center name
2023-03-02T11:31:34Z
INSDC last update
public
INSDC status
ERS14713882
SRA accession
E-MTAB-12700:ATGL-KO
Submitter Id
14
week
age
ArrayExpress
broker name
house mouse
common name
Protocols: After mouse sacrifice hearts were excised and retrograde perfused with first 5ml Heparin/PBS solution followed by 1ml PBS. Perfused hearts were place in OCT, snap-frozen in pre-cooled isopentane (-30 to -40°C) and stored at -80°C until further use. 10µm thick tissue sections were cut in a cryostat at -20°C chamber temperature and -10°C object head temperature and placed at the 10X Visium Gene Expression slide. Prepared Visium slides were used as input for the Visium Spatial Gene Expression workflow v1 (10X Genomics, Pleasanton, USA) according to the manufacturer’s instructions. Tissue permeabilization was carried out for 12 minutes Library construction was performed to manufacturer’s instructions, the Visium Spatial Gene Expression workflow (10X Genomics, Pleasanton, USA).
description
adult
developmental stage
iAdipoQCre: het, ATGL fl/fl
genotype
283075
individual
ischemia reperfusion injury
injury
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
heart
organism part
Mus musculus
scientific_name
male
sex
B6.129-Pnpla2tm1Eek/J Tg(Adipoq-cre/ERT2)1Soff/J
strain
ATGL-KO
title