SAMN46760195
External Id
Cedars-Sinai Medical Center
INSDC center name
2025-03-01T00:53:34.570Z
INSDC first public
2025-03-01T00:53:34.570Z
INSDC last update
SRS24046728
INSDC secondary accession
live
INSDC status
Metagenome or environmental
NCBI submission model
Metagenome.environmental.1.0
NCBI submission package
SRS24046728
SRA accession
2017
collection_date
Fecal sample_4
description
USA: California, Los Angeles
geo_loc_name
Mus musculus
host
Fecal pellet
isolation_source
34.076348 N 118.381189 W
lat_lon
mouse metagenome
http://purl.obolibrary.org/obo/NCBITaxon_1441287
organism
Not collected
ref_biomaterial
Not collected
rel_to_oxygen
Fecal pellets of SPF mice were collected at the beginning of the experiment (baseline) and 2 weeks (day 14) after LCWE injection. DNA for bacterial sequencing was extracted from fecal pellets (~50mg) per sample were homogenized, incubated with lyticase, bead beat, and DNA was extracted with the QIAamp DNA mini kit and 16S rRNA sequencing performed at Cedars-Sinai Applied Genomics, Computation, and Translational (AGCT) Core.
samp_collect_device
Fecal pellets of SPF mice were collected at the beginning of the experiment (baseline) and 2 weeks (day 14) after LCWE injection. DNA for bacterial sequencing was extracted from fecal pellets (~50mg) per sample were homogenized, incubated with lyticase, bead beat, and DNA was extracted with the QIAamp DNA mini kit and 16S rRNA sequencing performed at Cedars-Sinai Applied Genomics, Computation, and Translational (AGCT) Core.
samp_mat_process
Not applicable
samp_size
Day 0_4
source_material_id
Baseline_Day0_4
title