SAMN36986299
External Id
Northwestern University Feinberg School of Medicine
INSDC center name
2025-03-01T00:52:54.073Z
INSDC first public
2025-03-01T00:52:54.073Z
INSDC last update
SRS18607112
INSDC secondary accession
live
INSDC status
Human
NCBI submission model
Human.1.0
NCBI submission package
SRS18607112
SRA accession
1 year
age
ATCC TIB-202
biomaterial_provider
THP-1
cell_line
Monocyte
cell_type
Unknown
collection_date
THP-1 SAMHD1 knock-out monoclonal cells (9-11) were subject to cytoplasmic-nuclear fractionation. DNA was extracted from the cytoplasmic fraction and used directly for deep sequencing. Library preparation was performed using the Plexwell Plus 24 Library Preparation Kit (Seqwell cat. PWP24) according to manufacturer’s specifications. 10pM of each library was run on an Illumina MiSeq, using MiSeq reagent kit v3 600 cycle (Illumina cat MS-102-3003).
description
Infant
dev_stage
Acute monocytic leukemia
disease
United Kingdom
geo_loc_name
THP1-monoclonal-SAMHD1-KO_9-11
isolate
Homo sapiens
http://purl.obolibrary.org/obo/NCBITaxon_9606
organism
cell culture
sample_type
male
sex
Blood: Monocyte
tissue
THP-1 SAMHD1 Knock-out clone 9-11
title