SAMN47143759
External Id
Jiangsu University
INSDC center name
2025-03-01T00:00:00Z
INSDC first public
2025-03-02T01:29:20.263Z
INSDC last update
SRS24230410
INSDC secondary accession
live
INSDC status
Viral
NCBI submission model
Virus.1.0
NCBI submission package
SRS24230410
SRA accession
2023-09/2024-01
collection_date
For viral metagenomic analysis, 10 cerebrospinal fluid (CSF) specimens were collected from patients with influenza A and B in Bozhou City, Anhui Province, between September 2023 and January 2024. The 10 CSF samples were pooled into one specimen pool, with each pool containing 10 CSF samples. The pooled samples were thoroughly mixed, resuspended in 1 mL of DPBS, and subjected to three rapid freeze-thaw cycles on dry ice. The supernatants were collected after centrifugation (10 minutes, 15,000 g) and filtered through a 0.45 um filter (Millipore) to remove eukaryotic and bacterial cell-sized particles. A 200 uL aliquot of the supernatant from the pool was treated with a nuclease enzyme mixture to reduce the concentration of free (non-viral encapsidated) nucleic acids. The remaining total nucleic acids were isolated using the QIAamp MinElute Virus Spin Kit according to the manufacturer's protocol. One library was constructed using the Nextera XT DNA Sample Preparation Kit (Illumina) and sequenced on the Illumina NovaSeq platform with a 150 bp paired-end sequencing strategy.
description
China:bozhou,Anhui
geo_loc_name
missing
host
missing
isolate
human cerebrospinalfluid
isolation_source
missing
lab_host
missing
lat_lon
Nodaviridae sp.
http://purl.obolibrary.org/obo/NCBITaxon_1955166
organism
Human metagenome isolated from CSF samples of patients with influenza A and B
title