2025-03-03
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA115084280
External Id
Protein Research Unit - PRU Laboratory of Microbiology - LM Department Biochemistry and Microbiology Ghent University
INSDC center name
2024-01-05T17:13:52Z
INSDC last update
public
INSDC status
ERS17700253
SRA accession
E-MTAB-13688:DFA35 - Sample 2
Submitter Id
ArrayExpress
broker name
genetically modified strain
cell_type
not collected
collection date
Protocols: Overnight liquid cultures of each isolate, B. cenocepacia wild type and each mutant, that were grown at 37°C while shaking at 200 rpm in SCFM, were transferred to fresh media until the mid-exponential phase was reached. The cultures were subsequently diluted to a standardized culture to OD600nm 0.4 in 250 ml glass flasks. These cultures were further incubated for 18 hours before centrifugation at 4°C and the obtained cell pellets stored at −80 °C for a maximum of 1 week. Cells were grown at 37°C while shaking at 200 rpm in synthetic CF medium (SCFM). RNA extraction was outsourced to GENEWIZ Europe (Leipzig, Germany). Library prep was outsourced to GENEWIZ Europe (Leipzig, Germany).
description
with the deletion of atsR (bcam0379) gene
genetic modification
deficient derivative of the worldwide ET-12 epidemic clones
genotype
not collected
geographic location (country and/or sea)
replicate in synthetic CF medium (SCFM), the most in-vivo-like culturing medium, at 37°C and 200 rpm.
growth condition
not applicable
isolate
Burkholderia cenocepacia
organism
Burkholderia cenocepacia
organism
whole organism
organism part
Burkholderia cenocepacia
scientific_name
DFA35
strain
DFA35 - Sample 2
title