2025-03-04
ENA first public
ERC000011
ENA-CHECKLIST
NMBU
INSDC center name
public
INSDC status
ERS22551567
SRA accession
E-MTAB-14671:SL7B
Submitter Id
5 weeks (at the start of experiment)
week
age
ArrayExpress
broker name
not collected
collection date
perennial ryegrass
common name
Protocols: Right after the last watering (at 9 a.m.), crown tissue samples were collected from three replicates of each genotype (control). The next six sampling points were performed on the 3rd, 4th, and 5th day of mild drought stress, in the morning (9 a.m.) and afternoon (mid-light cycle, at 4 p.m.). Lastly, plants tissue was sampled 2 hrs after rewatering. Plnats were grown in commercially availiable substrate for 5 week old plants (25-30 tillers) in greenhouse. They were then transferred to growth chambers (16/8 hrs light/dark at 25 C) an kept for 4 days with normal watering to acclimate to new environment. RNA extraction was carried out using Qiagen RNeasy Plant Mini kit The libraries were prepared according to the TruSeq RNA Sample Prep Kit v2 (Illumina, San Diego, CA, USA)
description
Young plant
developmental stage
Sensitive
drought_phenotype (based on biomass)
3575 (ecotype, Origin=Lithuania)
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Lolium perenne
organism
Lolium perenne
organism
Crown tissue
organism part
2
replicate
Lolium perenne
scientific_name
D5R (105 hrs)
stimulus
SL7B
title