2025-03-09
ENA first public
ERC000011
ENA-CHECKLIST
Deptartment of Gynecology and Obstetrics University Medical Center Göttingen
INSDC center name
public
INSDC status
ERS18458718
SRA accession
E-MTAB-13917:Exp1_HeLa_siRNF40_3
Submitter Id
ArrayExpress
broker name
HeLa
cell_line
cervical cancer
cell_type
not collected
collection date
human
common name
Protocols: HeLa cells were grown in MEM medium supplemented with 10% fetal bovine serum, 100 units/ml penicillin and 100 μg/ml streptomycin at 37°C and 5% CO2. siRNA transfections were performed using DharmaFECT 1 Transfection Reagent (Horizon Discovery Ltd.) according to the manufacturer’s instructions. RNA was extracted usiung EXTRAzol® Lysis Reagent (BLIRT) according to the manufacturer's protocol. Libraries were prepared using TrueSeq™ V2 RNA-Seq Library Prep Kit (Illumina®) after verifying the RNA integrity on an agarose gel. Library quality was assessed using an Agilent Bioanalyzer 2100 (High Sensitivity DNA Kit).
description
adult
developmental stage
cervical cancer
disease
siRNF40
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
uterine cervix
organism part
Homo sapiens
scientific_name
Exp1_HeLa_siRNF40_3
title