2025-03-14
ENA first public
ERC000011
ENA-CHECKLIST
Faculty of Health & Life Sciences, University of Liverpool
INSDC center name
public
INSDC status
ERS23771031
SRA accession
E-MTAB-14888:2nd WT osteoblasts
Submitter Id
2.5
age
ArrayExpress
broker name
osteoblast
cell_type
not collected
collection date
house mouse
common name
Protocols: Primary osteoblasts were cultured from bone chips of genotyped 2.5 months mice. When cells were ready, osteoblasts were replated to 12 well plate for mRNA extraction. Bone chips were used for anotehr explants culture. When cells were ready, Trizol method were used for cells digestion. mRNA was extracted and purified with chloroform, isopropanol and ethanol. Dual-indexed, strand-specific RNASeq libraries from total RNA, using NEBNext polyA selection and Ultra II Directional RNA library preparation kits.
description
adult
developmental stage
normal
disease
Prx1-CreWT HuRfl/fl
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
long bone
organism part
Mus musculus
scientific_name
2nd WT osteoblasts
title