2025-03-17
ENA first public
ERC000011
ENA-CHECKLIST
Institut Pasteur, Paris
INSDC center name
public
INSDC status
ERS22576221
SRA accession
E-MTAB-14695:s16
Submitter Id
ArrayExpress
broker name
not collected
collection date
malaria parasite P. falciparum
common name
Protocols: P. falciparum sporozoites isolated from the salivary glands of infected mosquitoes were activated at 37°C in DMEM/F-12 medium supplemented with 10% FBS, 10 µM CD36 peptide, or both, or kept at 4°C to prevent activation. Each sample contained approximately 250,000 to 500,000 sporozoites. After 1 hour, sporozoites were spun down for 3 minutes at 13,000 rpm and resuspended in TRIzol reagent (Thermo Fisher Scientific) for RNA extraction. RNA was obtained by chloroform extraction. Total RNA was treated with DNase and purified using the RNeasy MinElute Cleanup Kit (QIAGEN). Library preparation was performed by Novogene (Cambridge, UK) using poly(A) mRNA enrichment.
description
not collected
geographic location (country and/or sea)
not applicable
isolate
Plasmodium falciparum
organism
Plasmodium falciparum
organism
whole organism
organism part
Plasmodium falciparum
scientific_name
FBS
stimulus
s16
title