2025-03-19
ENA first public
ERC000011
ENA-CHECKLIST
MPI for Biology Tuebingen
INSDC center name
public
INSDC status
ERS23282485
SRA accession
Tsu-0_met1_01
Submitter Id
not applicable
collection date
thale cress
common name
DNA extracted from rosettes of a single 35-days-old individual of a CRISPR-induced homozygous met1 mutant of accession Tsu-0 (ecotype_ID: 7373)
description
Japan
geographic location (country and/or sea)
34.43
geographic location (latitude)
136.31
geographic location (longitude)
growth chamber
growth facility
Seeds were germinated on soil and stratified in darkness at 4C for seven days, grown for 2 weeks in a growth chamber (CU-36L5; Percival Scientific, Wertingen, Germany) on half-strength MS agar plates supplemented with 1% w/v sucrose in long day conditions (16 h light) at 23C under 110 umol m-2 s-1, after which they were transferred to soil in long day conditions at 23C and 65% relative humidity under 110-140 umol m-2 s-1  light provided by Philips GreenPower TLED modules (Philips Lighting GmbH, Hamburg, Germany). To reduce starch accumulation, plants were placed into darkness for 24 h before harvesting.
isolation and growth condition
5
number of replicons
Arabidopsis thaliana
organism
vegetative
plant developmental stage
half-strength MS agar + soil
plant growth medium
Rosettes
plant structure
diploid
ploidy
healthy
sample health state
Arabidopsis thaliana
scientific_name
Tsu-0 met1
subspecific genetic lineage name
ecotype
subspecific genetic lineage rank
Tsu-0 met1 mutant 01
title