2025-03-20
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114264639
External Id
Nencki Institute of Experimental Biology
INSDC center name
2023-08-14T14:20:20Z
INSDC last update
public
INSDC status
ERS16253578
SRA accession
E-MTAB-13259:Mandy6_1
Submitter Id
ArrayExpress
broker name
Mandy6
cell_line
astrocyte
cell_type
not collected
collection date
chimpanzee
common name
Protocols: Cells were detached with Accutase, counted and crosslinked with 1% (final) formaldehyde in their medium. Reaction was quenched with 0.125M (final) glycine, cell pellets were washed with PBS, snap frozen and stored in -80 freezer. Pellet of 10 million cells was used for each CTCF ChIP experiment. iAstrocytes were grown on hESc-qualified Matrigel (Corning)-coated plates, in Astrocyte Medium (ScienCell). Seeding density was 15,000 cells/sq. cm. Hi-C experiments were performed as described as describer before (Rao et al., 2014) Hi-C libraries were generated using NEBNext Ultra II DNA Library Kit, according to manufacturer's instructions
description
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Pan troglodytes
organism
Pan troglodytes
organism
organism part
induced pluripotent stem cell
progenitor cell type
Pan troglodytes
scientific_name
female
sex
Mandy6_1
title