2025-03-20
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114262069
External Id
Nencki Institute of Experimental Biology
INSDC center name
2023-08-14T10:26:20Z
INSDC last update
public
INSDC status
ERS16251008
SRA accession
E-MTAB-13252:SandraA_1
Submitter Id
ArrayExpress
broker name
SandraA
cell_line
astrocyte
cell_type
not collected
collection date
chimpanzee
common name
Protocols: Cells (iAstrocytes) were detached with Accutase, counted and 500,000 cells were lysed with TRI-Reagent (Sigma). iAstrocytes were grown on hESc-qualified Matrigel (Corning)-coated plates, in Astrocyte Medium (ScienCell). Seeding density was 15,000 cells/sq. cm. RNA isolation was isolated using Direct-Zol RNA Isolation kit (ZYMO), according to the manufacturer's instructions. 1 ug of RNA (RIN > 8) was used for library constructions. Libraries were prepared using KAPA HyperPrep Kit, according to manufacturer's instructions. UDI/UMI (itDNA) indexes were used. Library quality was assessed with 4200 TapeStation System, High Sensitivity D1000 ScreenTape (Agilent).
description
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Pan troglodytes
organism
Pan troglodytes
organism
organism part
induced pluripotent stem cell
progenitor cell type
Pan troglodytes
scientific_name
female
sex
SandraA_1
title