SAMN47318259
External Id
Columbia University
INSDC center name
2025-03-22T00:39:58.613Z
INSDC first public
2025-03-22T00:39:58.613Z
INSDC last update
SRS24333532
INSDC secondary accession
live
INSDC status
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
SRS24333532
SRA accession
RNA_Th2_DKO_ns_rep2
Sample_ID
8 weeks
age
p65Tr/Tr Relfl/fl C57BL/6
breed
CD4+ T helper 2
cell_type
Vincent Guichard
collected_by
not collected
collection_date
not applicable
cultivar
not applicable
dev_stage
not applicable
ecotype
p65 Truncated - Rel KO
genotype
USA
geo_loc_name
CD4n were then sorted as live CD4+ CD44Low/- and then differentiated into Th2 cells on plates coated with 1 mg/mL anti-CD3/anti-CD28 antibodies in the presence of 1 mg/mL anti-IFNg (eBioscience) and 10 ng/mL IL-2/IL-4 (Peprotech) for 4 days, and then expanded for 3 days in the presence of IL-2 and IL-4 (10 ng/mL). To delete NF-kB subunit alleles, Th2 cells were incubated for 45 min at 37 °C in HBSS medium containing the TAT-CRE recombinase (50 mg/mL) (Merck Millipore). Transduction was stopped by washing cells in growth media before returning to culture. After 2 days, Th2 NF-kB-KO cells were sorted by FACS as CD4+GFP+ and cultured for 2 more days with IL-2 10 mg/mL before stimulation.
growth_protocol
not applicable
isolate
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
in-vitro culture
sample_type
male
sex
house mouse
strain
Lymphe node
tissue
Model organism or animal sample from Mus musculus
title
none
treatment