SAMN41585184
External Id
Stanford University
INSDC center name
2025-03-24T00:50:16.973Z
INSDC first public
2025-03-24T00:50:16.973Z
INSDC last update
SRS21461714
INSDC secondary accession
live
INSDC status
70nt DNA template input
Input DNA template
no
Input RNA primer
Metagenome or environmental
NCBI submission model
Metagenome.environmental.1.0
NCBI submission package
3h
Reaction time
SRS21461714
SRA accession
2023-06-08
collection_date
USA: California, Stanford
geo_loc_name
Homo sapiens
host
Generated in in vitro reactions with hmtRNAP and DNA oligonucleotide input, 3h timepoint
isolation_source
37.43326749 N 122.17495671 W
lat_lon
synthetic metagenome
http://purl.obolibrary.org/obo/NCBITaxon_1235509
organism
In vitro reactions were run with recombinantly expressed and purified preparations of human mitochondrial RNA polymerase with DNA oligonucleotide input. This reaction sample was quenched after 3 hours of reaction time. DragonRNA was isolated using phenol:chloroform extraction followed by ethanol precipitation.
samp_collect_device
Libraries were prepared following DruSeq, a modification of the Illumina TruSeq Small RNA protocol with the addition of polyethylene glycol.
samp_mat_process
Human mitochondrial RNA polymerase in vitro reactions with DNA oligonucleotide input 3 hour timepoint
title