SAMN45176147
External Id
Stanford University
INSDC center name
2025-03-24T00:50:17.220Z
INSDC first public
2025-03-24T00:50:17.220Z
INSDC last update
SRS23445425
INSDC secondary accession
live
INSDC status
Metagenome or environmental
NCBI submission model
Metagenome.environmental.1.0
NCBI submission package
SRS23445425
SRA accession
2024-07-22
collection_date
USA: California, Stanford
geo_loc_name
Homo sapiens
host
Generated in in vitro reactions with hmtRNAP and DNA oligonucleotide input 73, 20min reaction timepoint
isolation_source
37.43326749 N 122.17495671 W
lat_lon
synthetic metagenome
http://purl.obolibrary.org/obo/NCBITaxon_1235509
organism
In vitro reactions were run with recombinantly expressed and purified preparations of RNA polymerase (RNAP) with DNA oligonucleotide input. This reaction sample was quenched after reaction time. DragonRNA was isolated using phenol:chloroform extraction followed by ethanol precipitation.
samp_collect_device
Products were NOT treated with DNase. Libraries were prepared following DruSeq, a modification of the Illumina TruSeq Small RNA protocol with the addition of polyethylene glycol.
samp_mat_process
DragonRNA generated from human mitochondrial RNA polymerase in vitro reactions 20 minute timepoint with DNA oligonucleotide input version 1 that has 2 unspecified bases
title