SAMN45176143
External Id
Stanford University
INSDC center name
2025-03-24T00:50:15.863Z
INSDC first public
2025-03-24T00:50:15.863Z
INSDC last update
SRS23445434
INSDC secondary accession
live
INSDC status
Metagenome or environmental
NCBI submission model
Metagenome.environmental.1.0
NCBI submission package
SRS23445434
SRA accession
2024-08-02
collection_date
USA: California, Stanford
geo_loc_name
Saccharomyces cerevisiae
host
Generated in in vitro reactions with Rpo41 and double stranded DNA oligonucleotide input 82, 84, and 85, 30min reaction timepoint
isolation_source
37.43326749 N 122.17495671 W
lat_lon
synthetic metagenome
http://purl.obolibrary.org/obo/NCBITaxon_1235509
organism
In vitro reactions were run with recombinantly expressed and purified preparations of RNA polymerase (RNAP) with DNA oligonucleotide input. This reaction sample was quenched after reaction time. DragonRNA was isolated using phenol:chloroform extraction followed by ethanol precipitation.
samp_collect_device
Products were NOT treated with DNase. Libraries were prepared following DruSeq, a modification of the Illumina TruSeq Small RNA protocol with the addition of polyethylene glycol.
samp_mat_process
DragonRNA generated from yeast mitochondrial RNA polymerase (Rpo41) in vitro reactions 30 minute timepoint with double-stranded DNA oligonucleotide input that uses a DNA template with a three-oligo system with a 3-base gap before a DNA primer
title