SAMN41585186
External Id
Stanford University
INSDC center name
2025-03-24T00:50:18.240Z
INSDC first public
2025-03-24T00:50:18.240Z
INSDC last update
SRS21461711
INSDC secondary accession
live
INSDC status
27nt DNA template input
Input DNA template
no
Input RNA primer
Metagenome or environmental
NCBI submission model
Metagenome.environmental.1.0
NCBI submission package
1min
Reaction time
SRS21461711
SRA accession
2023-06-08
collection_date
USA: California, Stanford
geo_loc_name
Saccharomyces cerevisiae
host
Generated in in vitro reactions with Rpo41 and DNA oligonucleotide input, 1min reaction timepoint
isolation_source
37.43326749 N 122.17495671 W
lat_lon
synthetic metagenome
http://purl.obolibrary.org/obo/NCBITaxon_1235509
organism
In vitro reactions were run with recombinantly expressed and purified preparations of yeast mitochondrial RNA polymerase (Rpo41) with DNA oligonucleotide input. This reaction sample was quenched after 1 minute of reaction time. DragonRNA was isolated using phenol:chloroform extraction followed by ethanol precipitation.
samp_collect_device
Libraries were prepared following DruSeq, a modification of the Illumina TruSeq Small RNA protocol with the addition of polyethylene glycol.
samp_mat_process
Yeast mitochondrial RNA polymerase in vitro reactions with DNA oligonucleotide input 1 minute timepoint
title