2025-03-28
ENA first public
ERC000011
ENA-CHECKLIST
University Medical Center Göttingen Department of Gynecology and Obstetrics
INSDC center name
public
INSDC status
ERS23745319
SRA accession
E-MTAB-14877:H_DMSO_2
Submitter Id
ArrayExpress
broker name
HCT116
cell_line
colorectal cancer cell
cell_type
not collected
collection date
human
common name
Protocols: RNA samples were generated as pools. In detail, two biological replicates, each consisting of two pooled biological replicates per treatment group (total n = 4 per group; DMSO, Ganet, RG-7388, combi) were analyzed. The cells were cultured in RPMI 1640 supplemented with 10 % fetal bovine serum, glutamine and penicillin/streptomycin in a humidified atmosphere at 37°C with 5 % CO2. HCT116 cells were treated for 24 hrs with DMSO, 50 nM Ganetespib, 1 µM RG-7388 or in combination. The TRIZOL reagent from Invitrogen has been used for RNA extraction. Library construction performed by NovogeneNEB Next® Ultra™ RNA Library Prep Kit Library quality control: Agilent bioanalyzer 2100, DNA high-sensitivity chip Concentration checked by qPCR.
description
adult
developmental stage
colorectal cancer
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
colon
organism part
Homo sapiens
scientific_name
DMSO
stimulus
H_DMSO_2
title