2025-03-30
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA115053720
External Id
Department II of Internal Medicine and Center for Molecular Medicine Cologne and CECAD, University of Cologne
INSDC center name
2023-12-21T10:51:03Z
INSDC last update
public
INSDC status
ERS17594031
SRA accession
E-MTAB-13659:SN7640170_9430
Submitter Id
96
hour
age
1
batch
ArrayExpress
broker name
not collected
collection date
Protocols: Synchronized worms were grown at basal conditions (20°C) and collected at their day 1 adult stage in Trizol (Invitrogen). Total RNA was extracted using RNeasy Mini spin column (QIAGEN). Concentration and purity of the RNA was measured by NanoDrop. polyA+ mRNA was isolated from total RNA with NEBNext Poly(A) mRNA Magnetics Isolation Module (New England Biolabs). RNA-Seq libraries were prepared with NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). Capillary electrophoresis (Agilent Bioanalyser and Agilent Tapestation) was used to assess quality and quantity at all steps. Libraries were quantified by fluorimeter, immobilized and processed onto a flow cell with a cBot (Illumina).
description
adult
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Caenorhabditis elegans
organism
Caenorhabditis elegans
organism
whole organism
organism part
Caenorhabditis elegans
scientific_name
C. elegans Bristol N2
strain
SN7640170_9430
title