2025-03-30
ENA first public
ERC000011
ENA-CHECKLIST
University of California, Irvine
INSDC center name
public
INSDC status
ERS20736220
SRA accession
E-MTAB-14303:WT_SAL2
Submitter Id
12
week
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: Brains were rapidly dissected 1 hour after the end of the progressive ratio session and frozen in 2-methylbutane on dry ice.  Bilateral tissue punches of the ventral striatum were obtained and rapidly homogenized in TRIzol (Invitrogen; Cat# 15596026). Total RNA was extracted following the manufacturer's recommendation. Total RNA was monitored for quality control using the Nanodrop absorbance ratios for 260/280nm and 260/230nm. Total RNA was monitored for quality control using the Agilent Bioanalyzer Nano RNA chip and Nanodrop absorbance ratios for 260/280nm and 260/230nm. Library construction was performed according to the Illumina TruSeq® Stranded mRNA Sample Preparation Guide. The input quantity for total RNA was 500ng and mRNA was enriched using oligo dT magnetic beads. The enriched mRNA was chemically fragmented for three minutes. First strand synthesis used random primers and reverse transcriptase to make cDNA. After second strand synthesis the ds cDNA was cleaned using AMPure XP beads and the cDNA was end repaired and then the 3’ ends were adenylated. Illumina barcoded adapters were ligated on the ends and the adapter ligated fragments were enriched by nine cycles of PCR. The resulting libraries were validated by qPCR and sized by Agilent Bioanalyzer DNA high sensitivity chip.
description
adult
developmental stage
Drd2 flox (WT)
genotype
not collected
geographic location (country and/or sea)
WT_SAL2
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
nucleus accumbens
organism part
Mus musculus
scientific_name
male
sex
C57BL6
strain
WT_SAL2
title