2025-03-31
ENA first public
ERC000011
ENA-CHECKLIST
Centre for Cancer Immunology Cancer Sciences University of Southampton
INSDC center name
public
INSDC status
ERS21414879
SRA accession
E-MTAB-14594:Sample 12
Submitter Id
ArrayExpress
broker name
PD
clinical response
not collected
collection date
human
common name
Protocols: Fresh cores of tumors cores collected in RPMI were minced into 1 mm3 pieces or smaller using a scalpel and added to Liberase™ DL (Roche, Basel) RPMI for 15 minutes at 37 oC on an orbital shaker at 250 rpm. The sample mixture was then passed through a 70 µm filter, washed four times, red cell lysis (Qiagen) undertaken, and washed twice more. Resultant single cell suspension was cryopreserved in 50% v/v human AB serum (Sigma, Burlington), 40% supplemented RPMI and 10% v/v DMSO (Sigma, Burlington). Total RNA was isolated and processed using the Maxwell® RSC simplyRNA Cells and simplyRNA Tissue kits (Promega, Madison) following manufacturer’s instructions, and quantified and measured with Bioanalyser (Agilent Biosystems, Santa Clara). Sample library preparation was performed and quality checked by the Oxford Genomics Centre (OGC).
description
adult
developmental stage
FL
disease
not collected
geographic location (country and/or sea)
Patient F on
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
lymph node
organism part
WTCHG_706596_70925164
sample_id
Homo sapiens
scientific_name
female
sex
anti-CD20
stimulus
Sample 12
title