2025-03-31
ENA first public
ERC000011
ENA-CHECKLIST
Centre for Cancer Immunology Cancer Sciences University of Southampton
INSDC center name
public
INSDC status
ERS21414058
SRA accession
E-MTAB-14585:Sample 8
Submitter Id
ArrayExpress
broker name
inferred cell type
cell_type
SD
clinical response
not collected
collection date
human
common name
Protocols: Fresh cores of tumors cores collected in RPMI were minced into 1 mm3 pieces or smaller using a scalpel and added to Liberase™ DL (Roche, Basel) RPMI for 15 minutes at 37 oC on an orbital shaker at 250 rpm. The sample mixture was then passed through a 70 µm filter, washed four times, red cell lysis (Qiagen) undertaken, and washed twice more. Resultant single cell suspension was cryopreserved in 50% v/v human AB serum (Sigma, Burlington), 40% supplemented RPMI and 10% v/v DMSO (Sigma, Burlington). scRNAseq of pre- and on-treatment biopsies were performed using the 10X Genomics (Pleasanton, California) Chromium Single Cell 3’ Library & Gel Bead Kit (chemistry version 3.1) as per protocol, from thawed single-cell suspensions. scRNAseq of pre- and on-treatment biopsies were performed using the 10X Genomics (Pleasanton, California) Chromium Single Cell 3’ Library & Gel Bead Kit (chemistry version 3.1) as per protocol, from thawed single-cell suspensions.
description
adult
developmental stage
FL
disease
not collected
geographic location (country and/or sea)
Patient D on
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
lymph node
organism part
GX88
sample_id
Homo sapiens
scientific_name
male
sex
anti-CD20
stimulus
Sample 8
title