SAMN47614204
External Id
Radboud University
INSDC center name
2025-03-28T00:00:00Z
INSDC first public
2025-03-28T08:02:01.876Z
INSDC last update
SRS24523710
INSDC secondary accession
live
INSDC status
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
SRS24523710
SRA accession
8-12 weeks
age
sampling
age_event
not applicable
anatomic_site
Charles River Laboratories
biomaterial_provider
not applicable
cell_isolation
On average, 1.8 million sequencing reads were obtained per sample
cell_number
not applicable
cell_phenotype
Microdissected zones of the spleen were lysed in 700 μl of lysis buffer (Analytik Jena, Hildesheim, Germany). Following RNA isolation (Analytik Jena), TCR β-chain transcripts were amplified independently in a two-step reaction according to the manufacturer’s protocol (patent no. 7,999,092; iRepertoire).
cell_processing_protocol
not applicable
cell_quality
True
cell_storage
T cell
cell_type
not applicable
cells_per_reaction
not applicable
collection_date
not applicable
collection_time_point_reference
not applicable
collection_time_point_relative
not applicable
disease
not applicable
disease_length
not applicable
disease_stage
not applicable
ethnicity
not applicable
geo_loc_name
healthy
health_state
not applicable
immunogen
M2
isolate
not applicable
link_type
not applicable
linked_subjects
not applicable
medical_history
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
not applicable
population
not applicable
prior_therapies
not applicable
race
organ harvest
sample_type
female
sex
False
single_cell
C57BL/6
strain
not applicable
study_group_description
false
synthetic
Spleen T cell zone
tissue
Five splenic cryosections (12µm) were lysed in 0.7ml lysis buffer (Analytik Jena)
tissue_processing
Model organism or animal sample from Mus musculus
title
not applicable
treatment