2025-03-31
ENA first public
ERC000020
ENA-CHECKLIST
Scotland's Rural College - Aberdeen Campus
INSDC center name
public
INSDC status
ERS18895808
SRA accession
ms00469
Submitter Id
Forward overhang: 5'-AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATT; Reverse overhang: 5'-GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATGACTATCTCGTATGCCGTCTTCTGCTTG
adapters
environmental system determined by an organism
broad-scale environmental context
N/A
chimera check software
controlled glasshouse
climate environment
2021-12-30
collection date
Spring Wheat grown in clay from Yorkshire
description
rhizosphere environment
environmental medium
Wheat Rhizosphere
experimental factor
United Kingdom
geographic location (country and/or sea)
51.8094
DD
geographic location (latitude)
0.3561
DD
geographic location (longitude)
3
months
host age
spring wheat
host common name
flowering
host life stage
Mulika
host subspecific genetic lineage
4565
host taxid
plant-associated environment
local environmental context
NA
multiplex identifiers
N/A
negative control type
https://www.neb.com/en-gb/protocols/2014/05/22/protocol-for-use-with-nebnext-ultra-dna-library-prep-kit-for-illumina-e7370
nucleic acid amplification
Qiagen(TM) Power soil gDNA extraction kit
nucleic acid extraction
soil metagenome
organism
98�C for 30 seconds; 10 cycles of 98�C for 10 seconds, 98�C for 75 seconds; 98�C for 5 minutes; Hold at 4�C
pcr conditions
NA
pcr primers
root
plant body site
N/A
plant product
synthetic community of three known bacterial species
positive control type
UK Crop Microbiome Cryobank
project name
Rothamsted Research Station
reference for biomaterial
agmicrobiomebase.org
relevant electronic resources
0.5
g
sample volume or weight for DNA extraction
soil metagenome
scientific_name
software
sequence quality check
Novaseq 6000
sequencing method
NA
target gene
SW-CY-YO-05
title