2025-03-31
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA113578002
External Id
IMPERIAL COLLEGE LONDON
INSDC center name
2023-06-07T16:21:00Z
INSDC last update
public
INSDC status
ERS15572916
SRA accession
E-MTAB-13038:SPFC020-D2
Submitter Id
45
year
age
ArrayExpress
broker name
not provided
collection date
human
common name
Protocols: Nasal curettage samples were collected at baseline, Days 1, 2, 3, 7, 10 and 14 post-inoculation and preserved in TRIzol. These were stored at -80°C until processing. Total RNA was extracted from nasal curettage samples using Qiagen RNeasy Mini kit (Qiagen, Hilden, Germany). Strand-specific RNA sequencing library was prepared by using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA). The enriched RNAs were fragmented for 8 minutes at 94 °C. The first strand and second strand cDNA were subsequently synthesized. The second strand of cDNA was marked by incorporating dUTP during the synthesis. cDNA fragments were adenylated at 3’ends, and indexed adapter was ligated to cDNA fragments. Limited cycle PCR was used for library enrichment.
description
adult
developmental stage
not provided
geographic location (country and/or sea)
SPFC020
individual
H3N2 influenza A virus
infect
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
nasal tissue
organism part
Homo sapiens
scientific_name
male
sex
SPFC020-D2
title