2025-03-31
ENA first public
ERC000020
ENA-CHECKLIST
SAMEA114349172
External Id
Scotland's Rural College - Aberdeen Campus
INSDC center name
2023-09-11T17:12:59Z
INSDC last update
public
INSDC status
ERS16338210
SRA accession
ms00279
Submitter Id
Forward overhang: 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG; Reverse overhang: 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG
adapters
environmental system determined by an organism
broad-scale environmental context
N/A
chimera check software
controlled glasshouse
climate environment
2021-12-30
collection date
Spring Wheat grown in clay from Buckinghamshire
description
rhizosphere environment
environmental medium
Wheat Rhizosphere
experimental factor
United Kingdom
geographic location (country and/or sea)
51.8094
DD
geographic location (latitude)
0.3561
DD
geographic location (longitude)
3
months
host age
spring wheat
host common name
flowering
host life stage
Mulika
host subspecific genetic lineage
4565
host taxid
plant-associated environment
local environmental context
N/A
multiplex identifiers
N/A
negative control type
https://emea.support.illumina.com/downloads/16s_metagenomic_sequencing_library_preparation.html
nucleic acid amplification
Qiagen(TM) Power soil gDNA extraction kit
nucleic acid extraction
soil metagenome
organism
95�C for 3 minutes; 25 cycles of 95�C for 30 seconds, 55�C for 30 seconds, 72�C for 30 seconds; 72�C for 5 minutes; Hold at 4�C
pcr conditions
Forward: 5' CCTACGGGNGGCWGCAG; Reverse: 5' GACTACHVGGGTATCTAATCC
pcr primers
root
plant body site
N/A
plant product
N/A
positive control type
UK Crop Microbiome Cryobank
project name
Rothamsted Research Station
reference for biomaterial
agmicrobiomebase.org
relevant electronic resources
0.5
g
sample volume or weight for DNA extraction
soil metagenome
scientific_name
software
sequence quality check
MiSeq
sequencing method
16S
target gene
SW-CY-BU-03
title