SAMN25898497
External Id
Chongqing Academy of Animal Science
INSDC center name
2025-03-31T00:50:16.320Z
INSDC first public
2025-03-31T00:50:16.320Z
INSDC last update
SRS12004774
INSDC secondary accession
live
INSDC status
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
SRS12004774
SRA accession
32 week
age
Kunming mice
breed
Fat deposition in the liver is a characteristic of geese that is controlled by multiple genes. However, little is known about the roles of nuclear architecture during fat deposition in geese. In this proposal, we focus on the key issue of chromatin architecture reorganization in the nuclei of goose liver cells and aim to explore the highly dynamic chromatin architecture reorganization-mediated transcription that occurs during fat deposition. We aim to generate a high-resolution map of the 3D chromatin interactome during fat deposition in goose liver using HiC, RNAseq and ATACseq methods. We will focus on the analyses of chromatin contact maps at various resolutions, which have previously revealed principles of hierarchical genome architecture, spanning chromosome territories, compartments, and topologically-associating domains to contact domains, loops, and other important contacts mediated by cis-regulatory elements. With the functional evaluation of the specific long-range sequence interactions that vary during fat deposition, we envision that our discoveries will provide new information for the regulation of adipose-associated gene expression in the 3D genome. 3D fluorescence in situ hybridization will be employed to detect chromatin architecture reorganization in the nucleus, and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein technology will investigate gene function in hepatocytes. Studies of chromatin architecture reorganization in hepatocytes may also help uncover the molecular basis that underlies economic traits in geese. This will be an important step towards maximizing the economic benefits in producing high-quality goose products for human society.
description
adult
dev_stage
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
female
sex
mice
strain
liver
tissue
Mouse fatty liver model for NALFD
title