2025-03-31
ENA first public
ERC000011
ENA-CHECKLIST
Dr. Modhumita Dasgupta, Mrs. Muneera Parveen A, ICFRE - Institute of Forest Genetics and Tree Breeding, Coimbatore, Tamil Nadu, Dr. Suma Arun Dev, Sharath Parammel, Dr. V.B. Sreekumar, Kerala Forest Research Institute, Peechi, Kerala
INSDC center name
public
INSDC status
ERS20621128
SRA accession
SAMIN0004889-Whole genome sequencing of Calamus brandisii -Male
Submitter Id
IBDC
broker name
2019-11-15
collection date
Genomic DNA was isolated from the leaflet of male plant using ArborEasy DNA isolation kit. DNA concentration and purity was estimated using NanoDrop ND-1000 UV-Vis Spectrophotometer and DNA was quantified using Qubit Fluorometer. Library construction was conducted using KAPA HyperPlus Kit to generate fragments of size of 300-500bp. The library was subsequently quantified using Qubit Fluorometer and size distribution was checked on Agilent 2100 Bioanalyzer. The library was sequenced on Illumina NovaSeq using 150 bp paired-end chemistry.
description
India
geographic location (country and/or sea)
8°37'25.48"N
geographic location (latitude)
77°14'43.65" E
geographic location (longitude)
Natural Forest
isolation and growth condition
Calamus brandisii
organism
Matured leaflets of the male plant
plant developmental stage
Male
plant sex
Leaflets
plant structure
Calamus brandisii
scientific_name
Whole genome sequencing of Calamus brandisii -Male
title