2025-04-01
ENA first public
ERC000011
ENA-CHECKLIST
Estación Experimental del Zaidín (CSIC)
INSDC center name
public
INSDC status
ERS18678366
SRA accession
E-MTAB-13940:wt_MMR_1
Submitter Id
ArrayExpress
broker name
not collected
collection date
Protocols: Cells were harvested, and cell pellets were immediately frozen in liquid nitrogen and conserved at -80ºC until RNA isolation. MMR The strains were grown on LB plates for 48 hours at 28ºC and resuspended in 3 ml of 10 mM MgCl2. Ten ml of MMR minimal medium (Robertsen et al., 1982) were inoculated and the OD660 adjusted to 0.05. The strains were grown in MMR minimal medium at 20ºC to an OD660=1.5 rRNA removal with Ribo zero magnetic kit was performed by Macrogen The cells were processed for RNA isolation using TRI Reagent®LS (Molecular Research Center, Cincinnati, OH, USA) according to the manufacturer instructions, except that the TRI Reagent was preheated at 70ºC and the lysis step was carried out at 65ºC. The RNA obtained was treated with RNase-free DNase I (Roche) followed by purification with RNeasy mini kit (Quiagen).RNA concentration was determined spectrophotometrically and its integrity was assessed by agarose gel electrophoresis. Illumina cDNA libraries were constructed by Macrogen
description
not collected
geographic location (country and/or sea)
MMR minimal medium
growth condition
not applicable
isolate
Pseudomonas syringae pv. tomato str. DC3000
organism
Pseudomonas syringae pv. tomato str. DC3000
organism
1
replicate
Pseudomonas syringae pv. tomato str. DC3000
scientific_name
wt_MMR_1
title