SAMN40596745
External Id
Fudan University
INSDC center name
2025-03-31T00:51:33.127Z
INSDC first public
2025-03-31T00:51:33.127Z
INSDC last update
SRS20848434
INSDC secondary accession
live
INSDC status
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
SRS20848434
SRA accession
NA
age
NA
collection_date
To systematically study the functions of metabolic genes in regulating anti-tumor immunity, we performed a series of in vivo metabolic-wide CRISPR screens in orthotopic lung tumor mice models. We constructed a metabolic-focused sgRNA library, established LLC-Cas9-library pools, and then performed in vivo CRISPR screens. We compared the PBS treated tumors in Rag1-/- mice or C57BL/6 mice with the input cell pool to check the functions of metabolic genes in tumor growth; we compared the PBS treated tumors in C57BL/6 mice with the PBS treated tumors in Rag1-/- mice to check the functions of metabolic genes in regulating anti-tumor immunity; we compared the anti-PD1 treated tumors in C57BL/6 mice with PBS treated tumors in C57BL/6 mice to check the functions of metabolic genes in modulating sensitivity to anti-PD1 treatment.
description
NA
geo_loc_name
synthetic construct
http://purl.obolibrary.org/obo/NCBITaxon_32630
organism
not applicable
sex
NA
strain
NA
tissue
MusMet Library
title