2025-04-01
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA115162461
External Id
Institute of Molecular Genetics of the Czech Academy of Sciences
INSDC center name
2024-01-29T22:13:26Z
INSDC last update
public
INSDC status
ERS17944238
SRA accession
E-MTAB-13747:715_Tcf4_HOMO
Submitter Id
15
week
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: Adult littermates of the Apc-min Rosa26-tdTomato Defa6-iCre mouse strain carrying the Tcf7l2-wt or Tcf7l2-floxed alleles were sacrificed by cervical dislocation. In these mice developing multiple intestinal tumors, cells expressing Defa6 producing constitutively active Cre recombinase are marked by endogenous expression of the red fluorescent protein tdTomato. Adenomas from the colon of these mice were isolated, and a suspension of single cells was prepared by dispase and collagenase cleavage. Cells were stained with the following antibodies: Hoechst33358, anti-CD45-PacificBlue, anti-CD31-PacificBlue, anti-EpCAM-FITC. Using fluorescence-activated cell sorting (FACS), we isolated Hoechst-/CD45-/CD31-/EpCAM+/tdTomato+ cells, i.e. Defa6+ cells with either wild-type Tcf4 or homozygous loss of the Tcf4 protein. Total RNA was isolated using RNeasy Micro Kit (Qiagen) as described in the manufacturer’s protocol. Libraries were prepared with a SMARTer® Stranded Total RNA-Seq – Pico Input Mammalian library preparation kit v2 (Takara).
description
adult
developmental stage
Apc-min/+; Rosa26-tdTomato; Tcf7l2-flox/flox; DefaiCre
genotype
not collected
geographic location (country and/or sea)
3
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
Apc-deficient colon adenoma
organism part
Mus musculus
scientific_name
male
sex
C57BL/6
strain
715_Tcf4_HOMO
title