SAMN40596735
External Id
Fudan University
INSDC center name
2025-03-31T00:51:33.113Z
INSDC first public
2025-03-31T00:51:33.113Z
INSDC last update
SRS20848435
INSDC secondary accession
live
INSDC status
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
SRS20848435
SRA accession
not applicable
age
Lewis lung carcinoma cell line
cell_line
not applicable
collection_date
To systematically study the functions of metabolic genes in regulating anti-tumor immunity, we performed a series of in vivo metabolic-wide CRISPR screens in orthotopic lung tumor mice models. We constructed a metabolic-focused sgRNA library, established LLC-Cas9-library pools, and then performed in vivo CRISPR screens. We compared the PBS treated tumors in Rag1-/- mice or C57BL/6 mice with the input cell pool to check the functions of metabolic genes in tumor growth; we compared the PBS treated tumors in C57BL/6 mice with the PBS treated tumors in Rag1-/- mice to check the functions of metabolic genes in regulating anti-tumor immunity; we compared the anti-PD1 treated tumors in C57BL/6 mice with PBS treated tumors in C57BL/6 mice to check the functions of metabolic genes in modulating sensitivity to anti-PD1 treatment.
description
not applicable
geo_loc_name
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
not determined
sex
not applicable
strain
Lung
tissue
MusMet Library
title