2025-04-01
ENA first public
ERC000011
ENA-CHECKLIST
Estación Experimental del Zaidín (CSIC)
INSDC center name
public
INSDC status
ERS18678365
SRA accession
E-MTAB-13940:wt_MMF_2
Submitter Id
ArrayExpress
broker name
not collected
collection date
Protocols: Cells were harvested, and cell pellets were immediately frozen in liquid nitrogen and conserved at -80ºC until RNA isolation. MMF The strains were grown on LB plates for 48 hours at 28ºC and resuspended in 3 ml of 10 mM MgCl2. Fifteen ml of LB medium were inoculated, the OD adjusted to an OD660 of 0.05, and the strains were grown at 20ºC to an OD660=0.5. Then,the cultures were washed three times with MMF (Huynh et al., 1989), adjusted to an OD660=0.5 and incubated for 3 hours at 20°C. rRNA removal with Ribo zero magnetic kit was performed by Macrogen The cells were processed for RNA isolation using TRI Reagent®LS (Molecular Research Center, Cincinnati, OH, USA) according to the manufacturer instructions, except that the TRI Reagent was preheated at 70ºC and the lysis step was carried out at 65ºC. The RNA obtained was treated with RNase-free DNase I (Roche) followed by purification with RNeasy mini kit (Quiagen).RNA concentration was determined spectrophotometrically and its integrity was assessed by agarose gel electrophoresis. Illumina cDNA libraries were constructed by Macrogen
description
not collected
geographic location (country and/or sea)
Type III inducing minimal medium (MMF)
growth condition
not applicable
isolate
Pseudomonas syringae pv. tomato str. DC3000
organism
Pseudomonas syringae pv. tomato str. DC3000
organism
2
replicate
Pseudomonas syringae pv. tomato str. DC3000
scientific_name
wt_MMF_2
title