SAMN39858261
External Id
York University
INSDC center name
2025-03-31T00:51:54.297Z
INSDC first public
2025-03-31T00:51:54.297Z
INSDC last update
SRS20413285
INSDC secondary accession
live
INSDC status
Invertebrate
NCBI submission model
Invertebrate.1.0
NCBI submission package
SRS20413285
SRA accession
Jesse Huisken
collected_by
2023-09
collection_date
One biological sample of fresh-frozen brain tissue of 6 adult Ceratina calcarata dwarf-eldest daughters (DED) dissociated with S2 Genomics Singulator 100 system. Samples were loaded into 10X Genomics Gel-beads-in-emulsion wells on Chromium Chips following their protocol for Single Cell 3-prime Reagent Kits (v3.1). After dissolving the gel beads, nuclei were lysed, and mRNA was reverse transcribed to cDNA. Using PCR, the cDNA was then amplified to create libraries. Libraries were checked for quality using the Agilent Bioanalyzer High Sensitivity chip. Paired-end reads 1 and 2 and sample index reads were then sequenced on a flow cell lane (L003, L004, L005, L006, L007 or L008) with an Illumina Novaseq X to create base call files. Base call files were converted to fastq files for read 1 (R1, 28 bp) and read 2(R2, 90 bp) using Illumina BCL Convert (v. 4.1.5).
description
Adult
dev_stage
Canada:Toronto
geo_loc_name
Nests: Q007, N011,N009,N006,M025,M024
isolate
Rhus typhina or Rubus sp. stem
isolation_source
Ceratina calcarata
http://purl.obolibrary.org/obo/NCBITaxon_156304
organism
female
sex
Brain
tissue
single-nucleus transcriptome of six Ceratina calcarata brains: adult females from DED1
title