2025-04-02
ENA first public
ERC000024
ENA-CHECKLIST
IOW Leibniz-Institut fur Ostseeforschung Warnemuende
INSDC center name
public
INSDC status
ERS23959498
SRA accession
181954:5029d317-867f-47ef-8dc3-679b6e5cb489
Submitter Id
GFBIO
broker name
2022-06-16
collection date
sequences were generated in mixed orientation, separate read files submitted for each orientation
                    (here: R1 file contains forward reads)
comment
Surface water sample taken at Muehlendamm (54.0841494398656, 12.151006424457755) on 2022-06-16
            (YYYY-MM-DD), part of sequencing batch 3.
description
freshwater biome [ENVO:00000873]
environment (biome)
fresh water [ENVO:00002011]
environment (feature)
freshwater river [ENVO:01000297]|microbial community [PCO:1000004]
environment (material)
water
environmental package
yes
environmental_sample
s08T2422_lib1
experiment_id
Germany
geographic location (country and/or sea)
0
m
geographic location (depth)
0
m
geographic location (elevation)
54.08414944
DD
geographic location (latitude)
12.15100642
DD
geographic location (longitude)
Muehlendamm
geographic location (region and locality)
mimarks-survey
investigation type
DNA was extracted from the samples using the magMAX Wastewater Kit (cat. nr. A52606) according to
                    the protocol using the KingFisher Flex system after 10 sec of bead beating with silica beads.
nucleic acid extraction
freshwater metagenome
organism
OTC-Genomics
project name
Samples were taken with a metal bucket and transported to the research institute in a glass
                    bottle on ice. 500 ml of the sample was filtered onto a 0.22 um pore-size polycarbonate filter
                    (Durapore PVDF Filter, 47 mm diameter, Darmstadt, Germany) with 300-400 mbar, shock frozen in liquid
                    nitrogen and stored in a test tube at -80 degrees Celsius until DNA extraction.
sample material processing
Surface water sample taken at Muehlendamm (54.0841494398656, 12.151006424457755) on 2022-06-16
                    (YYYY-MM-DD), part of sequencing batch 3.
sample_description
freshwater metagenome
scientific_name
true
submitted to insdc
s08T2422
title
water
water environmental package