2025-04-03
ENA first public
ERC000011
ENA-CHECKLIST
PhD student
INSDC center name
public
INSDC status
ERS20522289
SRA accession
E-MTAB-14216:23DMSO_AD07
Submitter Id
ArrayExpress
broker name
primary cell line
cell_line
chondrocyte
cell_type
AD07
coculture attribute
not collected
collection date
human
common name
Protocols: Primary human chondrocytes were extracted from OA cartilage following total knee replacement surgery. Cartilages were sliced, washed, digested with Pronase E for 1h and then digested with Collagenase II overnight. The next day, chondrocytes were seeded at 3x10^5 cells/cm² in flasks in DMEM, 10%FBS, 1% L-Gln , 1%PS and were allowed to stand for 15 to 21 days, depending on proliferation rate of the donor. P0 OA chondrocytes were then seeded at 6x10^4 cells/cm² for 1 week and P1 OA chondrocytes were then stored in liquid nitrogen before cocultures. Total RNA was isolated using mini RNeasy kit (Qiagen) with an on-column DNAse I digestion step, according to the manufacturer instructions.The quantity and quality of extracted RNA was evaluated using the NanoDrop One spectrophotometer and the Agilent 2100 BioAnalyzer. Libraries were constructed by the Beijing Genomics Institute (BGI) on a DNBSEQ Eukaryotic Stranded Transcriptome library preparation for Stranded mRNA Library. All RNA integrity numbers were above 9.
description
adult
developmental stage
osteoarthritis, knee
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
cartilage
organism part
Homo sapiens
scientific_name
23DMSO_AD07
title