2025-04-03
ENA first public
ERC000011
ENA-CHECKLIST
Umeå University
INSDC center name
public
INSDC status
ERS23821665
SRA accession
E-MTAB-14943:Sample 43
Submitter Id
ArrayExpress
broker name
PSC5
cell_line
pancreatic stellate cell
cell_type
not collected
collection date
house mouse
common name
6 days
culture time
Protocols: Co-cultures of  pancreatic stellate cells and tumour organoids were dissociated to single with dispase and trypsin. Cells were stained with DAPI for viability and with MHCII antibody. PSC were isolated based on mKate2 fluorescence and MHCII expression.  500 cells were sorted directly into lysis buffer without RNA extraction Cell lysates were reverse transcribed directly in the lysis buffer as per the Smart-Seq2 protocol. Libraries were generated using nextera technique as per Smart-Seq2 protocol.
description
adult
developmental stage
PDAC
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
F12 advanced medium supplemented with  HEPES, Glutamine and Penicillin-Streptomycin
growth condition
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
pancreas
organism part
Mus musculus
scientific_name
C57B6
strain
Sample 43
title