2025-04-03
ENA first public
ERC000011
ENA-CHECKLIST
Umeå University
INSDC center name
public
INSDC status
ERS23821395
SRA accession
E-MTAB-14940:Sample 12
Submitter Id
35
week
age
ArrayExpress
broker name
All
cell_type
not collected
collection date
house mouse
common name
Protocols: Tumours were dissociated to single cell with collagenase D and liberase DL. Cells were stained with antibodies against CD45, Pdpn, and EpCAM. DAPI was used to select live cells Equal number of immune cells, fibroblasts epithelial cells and other cells types were collected by flow cytometry. 15 000 cells were used per lane in the 10x Chromium cartridge.  Mice with tumor diameter ~8-10 mm (8-12 weeks after transplantation) were recruited randomly in MSA-2 arm or the vehicle control arm. The drug regime included oral gavage of 80 mg/kg MSA-2 (MedChemExpress, HY-136927) or vehicle control (50% PEG300 (Sigma-Aldrich, 202371) in 0.9% NaCl) on day 0 and day 5. On day 6, mice were euthanized by CO2, and tumors were harvested and processed for single-cell RNA sequence profiling.  Cells were captured with the 10x chromium v3 protocol without RNA extraction Library were constructed with the 10x Chromium v3 dual index protocol following the manufacturer's instructions
description
adult
developmental stage
PDAC
disease
wild type genotype (KRAS/TP53 for tumour cells)
genotype
not collected
geographic location (country and/or sea)
4934
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
pancreas
organism part
Mus musculus
scientific_name
NA
sex
C57B6
strain
Sample 12
title