2025-04-03
ENA first public
ERC000011
ENA-CHECKLIST
Umeå University
INSDC center name
public
INSDC status
ERS23823208
SRA accession
E-MTAB-14946:Sample 96
Submitter Id
Run4
batch
ArrayExpress
broker name
PSC4
cell_line
Pancreatic stellate cells
cell_type
not collected
collection date
house mouse
common name
Mono-culture
culture type
Protocols: Co-cultures of  pancreatic stellate cells and tumour organoids were dissociated to single with dispase and trypsin. Cells were stained with DAPI for viability and with MHCII antibody. PSC were isolated based on mKate2 fluorescence and MHCII expression.  500 cells were sorted directly into lysis buffer without RNA extraction Cell lysates were reverse transcribed directly in the lysis buffer as per the Smart-Seq2 protocol. Libraries were generated using nextera technique as per Smart-Seq2 protocol.
description
adult
developmental stage
PDAC
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
C57B6
organism part
pancreas
organism part
Mus musculus
scientific_name
Sample 96
title