2025-04-03
ENA first public
ERC000011
ENA-CHECKLIST
PhD student
INSDC center name
public
INSDC status
ERS20522276
SRA accession
E-MTAB-14215:AD10_CTRL_RA10
Submitter Id
ArrayExpress
broker name
primary cell line
cell_line
adipose tissue derived mesenchymal stem cell
cell_type
not collected
collection date
human
common name
Protocols: Adipose-derived Mesenchymal Stromal Cells were lysed using RLT lysis buffer from RNAeasy kit  Total RNA was isolated using mini RNeasy kit (Qiagen) with an on-column DNAse I digestion step, according to the manufacturer instructions. The quantity and quality of extracted RNA was evaluated using the NanoDrop One spectrophotometer and the Agilent 2100 BioAnalyzer. Libraries were constructed by the Beijing Genomics Institute (BGI) on a DNBSEQ Eukaryotic Stranded Transcriptome library preparation for Stranded mRNA Library. All RNA integrity numbers were above 9.
description
adult
developmental stage
normal
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
adipose tissue
organism part
Homo sapiens
scientific_name
unstimulated
stimulus
AD10_CTRL_RA10
title