2025-04-04
ENA first public
ERC000024
ENA-CHECKLIST
Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research
INSDC center name
public
INSDC status
ERS22360971
SRA accession
208517:997887e8-44d4-4455-ad04-e48cd0e8d143
Submitter Id
fjord [ENVO:00000039]
broad-scale environmental context
GFBIO
broker name
2023-02-04
collection date
sequences were generated in mixed orientation, separate read files submitted for each orientation (here: R1 file contains forward reads)
comment
GAM21
cruise_station
21.3
m
depth
water sample, size fractions 0.2-0.8, 0.8-3, and 3-10 micrometer were pooled with equal volumes, sampled with CTD during cruise SO296/2, project MAPUCHE, at depth 20m, CTD cast SO2962_GAM21_045_10_CTD_0040
description
0
m
elevation
estuarine water [ENVO:01000301]
environmental medium
water
environmental package
yes
environmental_sample
GAM21_d20_lib1
experiment_id
Chile
geographic location (country and/or sea)
-51.799222
DD
geographic location (latitude)
-72.840528
DD
geographic location (longitude)
Patagonian fjord Golfo Almirante Montt
geographic location (region and locality)
mimarks-survey
investigation type
microbial community [PCO_1000004]
local environmental context
pooled
note_filter_size
QIAGEN  AllPrep DNA/RNA Mini Kit (Cat. No.: 80204)
nucleic acid extraction
seawater metagenome
organism
SO296/2_38-1
pangea_event_label
FWD: CCTAYGGGRBGCASCAG REV:GGACTACNNGGGTATCTAAT
pcr primers
MAPUCHE
project name
CTD free flow sample, filtration through 3, 0.8 and 0.2 microM, shock freezing with liquid nitrogen, storage at -80  degrees C; later pooling of the 3 size fractions
sample material processing
water sample, size fractions 0.2-0.8, 0.8-3, and 3-10 micrometer were pooled with equal volumes, sampled with CTD during cruise SO296/2, project MAPUCHE, at depth 20m, CTD cast SO2962_GAM21_045_10_CTD_0040
sample_description
seawater metagenome
scientific_name
Illumina MiSeq
sequencing method
SO2962_GAM21_045_10_CTD_0040
station_cast_id
true
submitted to insdc
V3-V4
target subfragment
GAM21_d20
title
184
m
total depth of water column
water
water environmental package