SAMN47633244
External Id
Kyung Hee University
INSDC center name
2025-04-03T00:00:00Z
INSDC first public
2025-04-04T15:02:33.156Z
INSDC last update
live
INSDC status
MIGS.ba
NCBI submission model
MIGS/MIMS/MIMARKS.human-oral
NCBI submission model
MIGS.ba.human-oral.6.0
NCBI submission package
2023-11-01
collection_date
Keywords: GSC:MIxS;MIGS:6.0
description
human-associated habitat
env_broad_scale
oral cavity
env_local_scale
Fastidious anaerobe agar (MB cell (cat. MB-F2169)), 0.25% mucin from porcine stomach (sigma cat. M2378), 10% Fetal bovine serum (Gibco cat. 16000044), 5% sheep blood
env_medium
South Korea: Seoul
geo_loc_name
Homo sapiens
host
Bacterial samples were collected by scraping the buccal mucosa using sterile cotton swabs. The swabs were then soaked in phosphate-buffered saline (PBS) to prepare a bacterial suspension, which was serially diluted. A 200 microL aliquot of the diluted suspension was plated onto Fastidious anaerobe agar supplemented with 5% sheep blood, 0.25% mucin, and 10% fetal bovine serum. The plates were incubated anaerobically at 37 C for 24 hours in an anaerobic chamber containing 10% CO?.., 10% H?.., and a nitrogen-balanced atmosphere.
isol_growth_condt
37.59 N 127.05 E
lat_lon
NA
num_replicons
Streptococcus sp. KHUD_013
http://purl.obolibrary.org/obo/NCBITaxon_3413147
organism
NA
ref_biomaterial
KHUD_013
strain
MIGS Cultured Bacterial/Archaeal sample from Streptococcus sp. KHUD_013
title