2025-04-10
ENA first public
ERC000011
ENA-CHECKLIST
The Azrieli Center for Stem Cells and Genetic Research Department of Genetics The Alexander Silberman Institute of Life Sciences The Hebrew University of Jerusalem Edmond J. Safra Campus, Givat Ram
INSDC center name
public
INSDC status
ERS21334480
SRA accession
E-MTAB-14543:FX-1
Submitter Id
ArrayExpress
broker name
FX52
cell_line
heterogenous composition from cerebral organoid differentation
cell_type
not collected
collection date
human
common name
Protocols: day 50 organoids were taken out of media, and immediately treated with Trizol and homogenized and stored in -80C until further extraction RNA extraction for cerebral organoids was performed using Trizol extraction. Organoids were lysed directly in Trizol (Invitrogen, Trizol, Cat #15596018). RNA was isolated according to the manufacturer’s instructions. Cells were directly sorted into 1mL of Trizol. RNA was extracted in the aqueous phase after addition of 200μL chloroform, precipitated with an equal volume of isopropanol and washed once in fresh 80% ethanol. The RNA pellet was re-suspended in 20μL ddH2O at 50C for 10 minutes and then kept on ice. RNA was stored at -20°C long term. PCR with Nextera overhangs
description
Day 50
developmental stage
Fragile X Syndrome
disease
FMR1 silencing by CGG expansion and hypermethylation
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
cerebral organoid
organism part
Homo sapiens
scientific_name
FX-1
title