2025-04-10
ENA first public
ERC000011
ENA-CHECKLIST
IP
INSDC center name
public
INSDC status
ERS21154759
SRA accession
E-MTAB-14532:WT2
Submitter Id
ArrayExpress
broker name
not collected
collection date
Protocols: samples were subcultured on columbia cystein agar at 37°C for 48 hours, and then cultured in 10 mL BHI broth firstly and 20 mL BHI broth secondly overnight at 37°C prior to isolation. All cultures have been perfomed in an anaerobic chamber (Coy Laboratory Products Inc, Grass Lake, MI, USA) (v/v: 90% N2, 5% H2, 5% CO2). Clostridium butyricum cultures were incubated until it reached an optical density (OD600) of 1.1 for isolation at mid-exponential phase. Total bacterial RNA was then extracted and purified using ZYMO Direct-zol™ RNA Miniprep Plus R2070 extraction kit and treated with DNAse I according to manufacturer’s instructions. A total of 100 ng RNA sample was used to construct each RNAseq library using Illumina stranded Total RNA Ligation Ribo0+ kit and subjected to ribosomal RNA (rRNA) depletion following manufacturer’s instructions
description
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Clostridium butyricum
organism
Clostridium butyricum
organism
Clostridium butyricum
scientific_name
WT2
title