2025-04-10
ENA first public
ERC000011
ENA-CHECKLIST
Department of Hematology  Erasmus MC
INSDC center name
public
INSDC status
ERS23831170
SRA accession
E-MTAB-14950:WT3_adultBM_LSK_SLAM
Submitter Id
8-12
week
age
ArrayExpress
broker name
hematopoietic stem cell
cell_type
not collected
collection date
house mouse
common name
Protocols: WT or GATA2 Animals were housed under specific-pathogen-free conditions. All experiments were performed in accordance with animal welfare regulations. 3x10^6 freshly isolated nucleated BM CD45.2+ cells from 65-week-old WT or Gata2+/- mice were transplanted via tail vein injection into lethally irradiated (10.5Gy) CD45.1+ WT recipient mice. RNA was isolated using Trizol  cDNA was smarter amplified
description
adult
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
lineage-cKit+Sca1+CD48-CD150+
immunophenotype
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
bone marrow
organism part
Mus musculus
scientific_name
male
sex
C57BL/6
strain
WT3_adultBM_LSK_SLAM
title