SAMN47869146
External Id
Vignan's Foundation for Science, Technology & Research
INSDC center name
2025-04-10T00:00:00Z
INSDC first public
2025-04-10T08:01:32.687Z
INSDC last update
live
INSDC status
MIGS.ba
NCBI submission model
MIGS/MIMS/MIMARKS.food-human_foods
NCBI submission model
MIGS.ba.food-human_foods.6.0
NCBI submission package
Commercial product obtained from pharmaceutical supply
coll_site_geo_feat
2025-01-20
collection_date
This study evaluated the probiotic properties of multi-antibiotic-resistant strains derived from Entromax. Four strains BCPS01, BCPS02, BCPS03, and BCPS04 were subjected to comprehensive phenotypic, biochemical, and molecular characterization, including antibiotic susceptibility testing. Key probiotic attributes such as tolerance to gastric juice, bile salts, and osmotic stresses were assessed alongside enzymatic activities like casein and starch hydrolysis. Antibiotic resistance profiles revealed that BCPS01, BCPS02, BCPS03, and BCPS04 exhibited resistance to chloramphenicol, rifampin, streptomycin, and tetracycline respectively. Phylogenetic analysis based on 16S rRNA sequencing indicated a close relationship with Shouchella clausii, while whole-genome BLAST analysis confirmed 100% similarity with Bacillus clausii ENTPro. The observed resistance to acidic conditions, bile salts, and gastric juice highlights critical probiotic characteristics, supporting the potential of these strains for probiotic applications.
description
soil
env_broad_scale
antibiotic exposure
env_local_scale
Capsule shell and probiotic powder from Entromax
env_medium
Probiotic
food_product_type
India: Hyderabad
geo_loc_name
Nutritional and dietary supplement
ifsac_category
in LB broth supplemented with sodium chloride concentrations ranging from 2% to 8%.
isol_growth_condt
17.4065 N 78.4772 E
lat_lon
2
num_replicons
Shouchella clausii
http://purl.obolibrary.org/obo/NCBITaxon_79880
organism
Khatri, I., Sharma, G. & Subramanian, S. Composite genome sequence of Bacillus clausii, a probiotic commercially available as Enterogermina and insights into its probiotic properties. BMC Microbiol 19, 307 (2019). https://doi.org/10.1186/s12866-019-1680-7
ref_biomaterial
Bacillus clausii
strain
MIGS Cultured Bacterial/Archaeal sample from Shouchella clausii
title