2025-04-15
ENA first public
ERC000011
ENA-CHECKLIST
Istituto di Ricerche Farmacologiche \"Mario Negri\" IRCCS
INSDC center name
public
INSDC status
ERS21115756
SRA accession
E-MTAB-14498:MCF7_shNR2F6_C_REP3
Submitter Id
ArrayExpress
broker name
MCF7
cell_line
breast invasive ductal carcinoma cell
cell_type
not collected
collection date
human
common name
Protocols: Before library preparation, RNA concentration was evaluated through Qubit™ RNA High Sensitivity Assay Kit (Invitrogen, Walthman, MA, USA) while RNA quality was established using 4200 Tapestation (Agilent Technologies, Santa Clara, CA, USA).  Protocol: miRNeasy Mini Kit. Following the TruSeq Stranded Total RNA protocol (Illumina, San Diego, CA, USA), at least 500 ng of RNA whose RIN value was between 7 and 9, were used for RNA sequencing. TruSeq Stranded Total RNA Sample Preparation Guide. Following the TruSeq Stranded Total RNA protocol (Illumina, San Diego, CA, USA), at least 500 ng of RNA whose RIN value was between 7 and 9, were used for RNA sequencing.
description
breast cancer
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
breast
organism part
Homo sapiens
scientific_name
MCF7_shNR2F6_C_REP3
title