SAMN46824717
External Id
Stazione Zoologica Anton Dohrn
INSDC center name
2025-04-19T06:40:18.320Z
INSDC first public
2025-04-19T06:40:18.320Z
INSDC last update
SRS24085537
INSDC secondary accession
live
INSDC status
Invertebrate
NCBI submission model
Invertebrate.1.0
NCBI submission package
SRS24085537
SRA accession
2023-10-18/2023-10-25
collection_date
255 m
depth
This BioProject presents the transcriptomic analysis of the deep-sea zoantharian Zibrowius primnoidus, collected at 255 m depth in the Sicily Channel, Italy, on 18 and 25 October 2023, to characterize its toxin repertoire using a bulk RNA-seq approach. Zibrowius primnoidus belongs to the order Zoantharia (Cnidaria) and is a deep-sea anthozoan with ecological and evolutionary significance, particularly in understanding venom diversity and molecular adaptations in mesophotic and deep-sea environments. A total of twelve polyps were preserved in RNA later to maintain RNA integrity. The samples were then washed in PBS and processed using a TRIzol-based RNA extraction protocol incorporating magnetic beads, which enhanced RNA recovery. After purification using the Mag-Bind Total Pure NGS kit, RNA yield was quantified at 563 ng/uL, and its integrity was assessed using a High Sensitivity RNA Screen Tape Assay kit on the TapeStation System. Library preparation was performed using the Zymo-Seq RiboFree Total RNA Kit, a method optimized for rRNA depletion, ensuring a comprehensive transcriptome representation. Sequencing was carried out on an Illumina NovaSeq 6000 platform in paired-end 150 bp mode, generating 90,741,564 raw reads. Primary bioinformatics processing included base calling, demultiplexing, and adapter masking using Illumina BCL Convert v3.9.31, ensuring high-quality sequence data for downstream analysis. Further computational analyses will focus on transcriptome assembly, functional annotation, and differential expression profiling to identify toxin-related genes and other key molecular pathways involved in deep-sea adaptation.
description
Colony
dev_stage
Italy: Sicily Channel, Mediterranean Sea
geo_loc_name
Zibrowius primnoidus polyps
host
polyps from a zoantharian colony
host_tissue_sampled
colonies
isolate
Zibrowius primnoidus
http://purl.obolibrary.org/obo/NCBITaxon_1590592
organism
polyps
tissue
Zibrowius primnoidus colony RNAseq sample from the Sicily Channel, Mediterranean Sea
title