characteristics.text: 2025-04-28
characteristics.characteristic: ENA first public
characteristics.text: ERC000024
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: IOW Leibniz-Institut fur Ostseeforschung Warnemuende
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS23958757
characteristics.characteristic: SRA accession
characteristics.text: 182875:5029d317-867f-47ef-8dc3-679b6e5cb489
characteristics.characteristic: Submitter Id
characteristics.text: GFBIO
characteristics.characteristic: broker name
characteristics.text: 2023-05-11
characteristics.characteristic: collection date
characteristics.text: sequences were generated in mixed orientation, separate read files submitted for each orientation
                      (here: R1 file contains forward reads)
characteristics.characteristic: comment
characteristics.text: Surface water sample taken at Muehlendamm (54.0841494398656, 12.151006424457755) on 2023-05-11
              (YYYY-MM-DD), part of sequencing batch 18.
characteristics.characteristic: description
characteristics.text: freshwater biome [ENVO:00000873]
characteristics.characteristic: environment (biome)
characteristics.text: fresh water [ENVO:00002011]
characteristics.characteristic: environment (feature)
characteristics.text: freshwater river [ENVO:01000297]|microbial community [PCO:1000004]
characteristics.characteristic: environment (material)
characteristics.text: water
characteristics.characteristic: environmental package
characteristics.text: yes
characteristics.characteristic: environmental_sample
characteristics.text: s08T1923_lib1
characteristics.characteristic: experiment_id
characteristics.text: Germany
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 0
characteristics.unit: m
characteristics.characteristic: geographic location (depth)
characteristics.text: 0
characteristics.unit: m
characteristics.characteristic: geographic location (elevation)
characteristics.text: 54.08414944
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: 12.15100642
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: Muehlendamm
characteristics.characteristic: geographic location (region and locality)
characteristics.text: mimarks-survey
characteristics.characteristic: investigation type
characteristics.text: DNA was extracted from the samples using the magMAX Wastewater Kit (cat. nr. A52606) according to
                      the protocol using the KingFisher Flex system after 10 sec of bead beating with silica beads.
characteristics.characteristic: nucleic acid extraction
characteristics.text: freshwater metagenome
characteristics.characteristic: organism
characteristics.text: OTC-Genomics
characteristics.characteristic: project name
characteristics.text: Samples were taken with a metal bucket and transported to the research institute in a glass
                      bottle on ice. 500 ml of the sample was filtered onto a 0.22 um pore-size polycarbonate filter
                      (Durapore PVDF Filter, 47 mm diameter, Darmstadt, Germany) with 300-400 mbar, shock frozen in liquid
                      nitrogen and stored in a test tube at -80 degrees Celsius until DNA extraction.
characteristics.characteristic: sample material processing
characteristics.text: Surface water sample taken at Muehlendamm (54.0841494398656, 12.151006424457755) on 2023-05-11
                      (YYYY-MM-DD), part of sequencing batch 18.
characteristics.characteristic: sample_description
characteristics.text: freshwater metagenome
characteristics.characteristic: scientific_name
characteristics.text: true
characteristics.characteristic: submitted to insdc
characteristics.text: s08T1923
characteristics.characteristic: title
characteristics.text: water
characteristics.characteristic: water environmental package