characteristics.text: SAMN44769579
characteristics.characteristic: External Id
characteristics.text: Clemson University
characteristics.characteristic: INSDC center name
characteristics.text: 2025-04-26T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2025-04-26T16:38:02.189Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS23226646
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Human
characteristics.characteristic: NCBI submission model
characteristics.text: Human.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS23226646
characteristics.characteristic: SRA accession
characteristics.text: Not applicable
characteristics.characteristic: age
characteristics.text: Andrei Alexandrov
characteristics.characteristic: biomaterial_provider
characteristics.text: Hela cell
characteristics.characteristic: cell_line
characteristics.text: 2017-10-18
characteristics.characteristic: collection_date
characteristics.text: Enriched sgRNA library obtained from the screen
characteristics.characteristic: description
characteristics.text: Not applicable
characteristics.characteristic: geo_loc_name
characteristics.text: Genomic DNA was isolated from FACS-isolated cells using phenol extraction. Sequences of guide RNAs were amplified from genomic DNA in two steps. First, a linear sgRNA amplification was performed using Herculase II DNA polymerase using 13 thermal cycles with a single sgRNA promoter-specific primer, RandomF (sequences of all primers are listed in Supplemental Data S4), and the following cycling parameters: 96C 20s, 63C 1min, 72C 90s. Then, the second PCR primer, RandomR, was added to the reaction and a regular PCR was performed for 35 cycles as follows: 96C 3min; 35 cycles of 96C 20s, 63C 1min, 72C 45s; 72C 10min; 4C.
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Not applicable
characteristics.characteristic: sex
characteristics.text: Not applicable
characteristics.characteristic: tissue
characteristics.text: Human sample from Homo sapiens
characteristics.characteristic: title