characteristics.text: SAMN37847277
characteristics.characteristic: External Id
characteristics.text: Vivian Cheung Lab, Life Sciences Institute
characteristics.characteristic: INSDC center name
characteristics.text: 2025-04-27T00:50:19.380Z
characteristics.characteristic: INSDC first public
characteristics.text: 2025-04-27T00:50:19.380Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS19169299
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Human
characteristics.characteristic: NCBI submission model
characteristics.text: Human.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS19169299
characteristics.characteristic: SRA accession
characteristics.text: neonatal
characteristics.characteristic: age
characteristics.text: Penn SBDRC
characteristics.characteristic: biomaterial_provider
characteristics.text: FB3386
characteristics.characteristic: cell_line
characteristics.text: foreskin
characteristics.characteristic: cell_subtype
characteristics.text: fibroblast
characteristics.characteristic: cell_type
characteristics.text: 2020-10-01
characteristics.characteristic: collection_date
characteristics.text: Design: PRO-seq library generated from neonatal foreskin fibroblasts. Cells were treated with 1 uM RHPS4 or vehicle for 24 hours; Drosophila S2 cell spike-ins were added. Biotin-labelled nascent RNA converted to cDNA and the product was amplified 22 cycles. Products between 150-300 bp (insert >70 bp) were PAGE purified then sequenced by the Illumina HiSeq 2500 instrument to a depth of >150 million reads.
characteristics.characteristic: description
characteristics.text: neonatal
characteristics.characteristic: dev_stage
characteristics.text: n/a
characteristics.characteristic: geo_loc_name
characteristics.text: n/a
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: cell culture (MEM+10% FBS)
characteristics.characteristic: sample_type
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: neonatal foreskin fibroblasts
characteristics.characteristic: tissue
characteristics.text: control fibroblasts
characteristics.characteristic: title
characteristics.text: DMSO for 24 hours
characteristics.characteristic: treatment