characteristics.text: SAMN44794969
characteristics.characteristic: External Id
characteristics.text: Clemson University
characteristics.characteristic: INSDC center name
characteristics.text: 2025-04-26T00:00:00Z
characteristics.characteristic: INSDC first public
characteristics.text: 2025-04-26T16:38:02.011Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS23241998
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Human
characteristics.characteristic: NCBI submission model
characteristics.text: Human.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS23241998
characteristics.characteristic: SRA accession
characteristics.text: Not applicable
characteristics.characteristic: age
characteristics.text: Andrei Alexandrov
characteristics.characteristic: biomaterial_provider
characteristics.text: Hela Cell
characteristics.characteristic: cell_line
characteristics.text: 2022-05-25
characteristics.characteristic: collection_date
characteristics.text: Knockout of ZCRB1.
characteristics.characteristic: description
characteristics.text: Not applicable
characteristics.characteristic: geo_loc_name
characteristics.text: Knockout of ZCRB1. Cell populations with same fluorescence compared with negative control of lentivirally-transduced cells expressing Cas9 and gene-specific sgRNAs (pAVA3250 targeting RBM7) were FACS-isolated. Extracted RNA was diluted to 50ng/ul and sent for cDNA library construction (without the use of poly(A) selection) and paired-end sequencing (100-bp paired-end reads) following the manufacturer's protocols (BGI-America, San Jose, CA). 60 millions clean reads received for each sample
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_9606
characteristics.characteristic: organism
characteristics.text: Not applicable
characteristics.characteristic: sex
characteristics.text: Not applicable
characteristics.characteristic: tissue
characteristics.text: ZCRB1-KO
characteristics.characteristic: title