characteristics.text: 2025-04-29
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: King's college London
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS24277388
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-13535_5:E135-GFP-pos-F2-1
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: germ cell
characteristics.characteristic: cell type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: Experimental F1 embryos were generated by crossing Xist+/∆ (B6) dams carrying Oct4-EGFP transgene, and CAST sires, producing F1 female (XX(Xist∆)) and males (XY) that were analyzed.   Following timed natural matings, F1 embryos were harvested daily at E10.5 to E16.5. Gonads were dissected and dissociated into single-cell suspension. EGFP fluorescence was used to isolate the fluorescently marked germ cells with FACS. Viable individual germ cells and somatic cells were sorted by FACS into 96-well plates containing 4µl lysis buffer. Please see 'nucleic acid library construction protocol'. Single-cell RNA-seq libraries were generated as described in Picelli et al., 2014, Nature Protocols. Full-length RNA-seq from single cells using SMART-seq2.
characteristics.characteristic: description
characteristics.text: E13.5
characteristics.characteristic: developmental stage
characteristics.text: F2
characteristics.characteristic: embryo_id
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: gonad
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: castaneus x C57B6Nj
characteristics.characteristic: strain
characteristics.text: E135-GFP-pos-F2-1
characteristics.characteristic: title