characteristics.text: 2025-04-30
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Cell and Development Biology, University College London
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS21119624
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-14510:S1_NL4
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: chicken
characteristics.characteristic: common name
characteristics.text: Protocols: Tissues (HH4- node sub-regions) were isolated from transgenic-GFP embryos using 30G syringe needles in sterile molecular grade PBS. Tissues were collected into RNAlater (Invitrogen, # AM7020). For each sample, tissues were collected from thirteen to seventeen embryos. RNA was extracted using the Micro Total RNA Isolation Kit (Invitrogen, # AM1931) and concentration and quality measured using Tapestation The NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (# E6420) was used for cDNA and library synthesis
characteristics.characteristic: description
characteristics.text: HH4-
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Gallus gallus
characteristics.characteristic: organism
characteristics.text: Gallus gallus
characteristics.characteristic: organism
characteristics.text: Hensen's node anterior left
characteristics.characteristic: sampling site
characteristics.text: Gallus gallus
characteristics.characteristic: scientific_name
characteristics.text: S1_NL4
characteristics.characteristic: title