characteristics.text: 2025-04-30
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Fraunhofer Institute for Molecular Biology and Applied Ecology (IME)
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS21156065
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-14536:Sample 3
characteristics.characteristic: Submitter Id
characteristics.text: 96
characteristics.unit: hour
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: zebrafish
characteristics.characteristic: common name
characteristics.text: Protocols: at the end of exposure, 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep-24 homogeniser (MP Biomedicals, Irvine, USA) at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to difenoconazole for 96 hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub-lethal concentrations of difenoconazole (high exposure and low exposure concentrations) in addition to untreated control groups. total RNA and Protein were extracted from the tissue lysate using using the NucleoSpin® RNA/Protein kit (Macherey & Nagel, Düren, Germany). according to the manufacturer’s protocol. Nanodrop 2000 spectrophotometer (Thermo Scientific) was used to measure and normalize the obtained RNA concentrations. The quality of the extracted RNA samples was checked using RNA 6000 Nano kit in 2100 Bioanalyzer System (Agilent, Santa Clara, USA). Samples were stored at -80°C.   RNA libraries were prepared from RNA extracts with RNA integrity number (RIN) values > 8 using poly(A) RNA purification and the TruSeq RNA Library Prep Kit v2 (Illumina, San Diego, USA)
characteristics.characteristic: description
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Danio rerio
characteristics.characteristic: organism
characteristics.text: Danio rerio
characteristics.characteristic: organism
characteristics.text: whole organism
characteristics.characteristic: organism part
characteristics.text: Danio rerio
characteristics.characteristic: scientific_name
characteristics.text: AB
characteristics.characteristic: strain
characteristics.text: T13
characteristics.characteristic: tank
characteristics.text: Sample 3
characteristics.characteristic: title