characteristics.text: 2025-04-30
characteristics.characteristic: ENA first public
characteristics.text: ERC000043
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Two strains of Saccharina latissima collected at Perharidy (near Roscoff, north Finistere, Brittany, France, January 2016, strain SLPER139) and Saint Guenole (south Finistere, Brittany, France, October 2015, strain SLSTG055) were crossed to produce the F1 hybrid parent F1.27.2. A bulk cross using gametophytes derived from F1.27.2 then generated 189 F2 sporophytes. Strain F1.27.2 and 129 F2 hybrids were cultivated on an open water rope system in Morlaix Bay in 2017. The remaining 60 F2 hybrids were cultivated in the laboratory in natural seawater with half-strength Provasoli enrichment in aerated 10 L bottles at 13�C. After harvesting tissue discs were excised from the blades and stored dessicated in silica gel for DNA extraction. ddRAD-seq libraries were constructed with a size-selected range of fragments of 350-800 bp (before addition of the barcodes/adaptateurs/index).
characteristics.characteristic: Further Details
characteristics.text: SBR
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS21377422
characteristics.characteristic: SRA accession
characteristics.text: Slat_PopF2_QTL
characteristics.characteristic: Submitter Id
characteristics.text: 17 mm blade disc
characteristics.unit: L
characteristics.characteristic: amount or size of sample collected
characteristics.text: Zofia Nehr
characteristics.characteristic: collected_by
characteristics.text: 2017-10-01
characteristics.characteristic: collection date
characteristics.text: 1
characteristics.unit: m
characteristics.characteristic: depth
characteristics.text: DNA samples extracted for ddRAD-seq analysis from 17 mm discs excised from Saccharina latissima strains SLPER139, F1.27.2 and 189 F2 progeny produced by selfing F1.27.2.
characteristics.characteristic: description
characteristics.text: Atlantic Ocean
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 48.709483
characteristics.unit: DD
characteristics.characteristic: geographic location (latitude)
characteristics.text: -3.953283
characteristics.unit: DD
characteristics.characteristic: geographic location (longitude)
characteristics.text: Either open water culture using seeded cotton strings attached to horizontal ropes attached to anchored bouys in Morlaix bay (strain F1.27.2 and 129 F2 hybrids) or laboratory cultivation in natural seawater with half-strength Provasoli enrichment in aerated 10 L bottles at 13�C (60 F2 hybrids).
characteristics.characteristic: growth condition
characteristics.text: Saccharina latissima
characteristics.characteristic: organism
characteristics.text: Excision of a 17 mm disc from the meristematic region of the blade.
characteristics.characteristic: sample collection method
characteristics.text: Silica gel
characteristics.characteristic: sample storage conditions
characteristics.text: 6 years
characteristics.characteristic: sample storage duration
characteristics.text: 20
characteristics.unit: °C
characteristics.characteristic: sample storage temperature
characteristics.text: Saccharina latissima
characteristics.characteristic: scientific_name
characteristics.text: SLPER139, F1.27.2 and 189 F2 progeny derived from F1.27.2
characteristics.characteristic: strain
characteristics.text: DNA from Saccharina latissima strains SLPER139, F1.27.2 and 189 F2 progeny
characteristics.characteristic: title