characteristics.text: 2025-04-30
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: SAMEA115057955
characteristics.characteristic: External Id
characteristics.text: UCL-Great Ormond Street Institute of Child Health
characteristics.characteristic: INSDC center name
characteristics.text: 2023-12-22T14:40:25Z
characteristics.characteristic: INSDC last update
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS17598451
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-13673:Sample 73
characteristics.characteristic: Submitter Id
characteristics.text: 11
characteristics.unit: week
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Human fetal tissue samples used for bulk-RNA seq were obtained with ethical approval and informed consent from the Human Developmental Biology Resource (http://www.hdbr.org).The age was estimated from foot length and knee-heel length in relation to standard growth data. Samples were karyotyped by quantitative PCR (chromosomes 13, 15, 16, 18, 21, 22 and X and Y) to determine the sex of the fetus. Samples were stored in -70C until use. Total RNA was extracted from human fetal tissue samples using the AllPrep DNA/RNA Mini Kit (Qiagen). cDNA libraries were prepared using the KAPA mRNA HyperPrep Kit (Roche) on a Hamilton Starlet robotic platform (Hamilton) and quality was analyzed using a Tapestation 4200 platform (Agilent technologies).
characteristics.characteristic: description
characteristics.text: 11wpc
characteristics.characteristic: developmental stage
characteristics.text: monosomy X
characteristics.characteristic: disease
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: fetal
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: 45,X
characteristics.characteristic: karyotype
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: placenta
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: 45,X
characteristics.characteristic: sex
characteristics.text: Sample 73
characteristics.characteristic: title