name: Ikba-HIGH
characteristics.text: 2025-05-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: NKI
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS23861868
characteristics.characteristic: SRA accession
characteristics.text: Ikba-HIGH
characteristics.characteristic: Submitter Id
characteristics.text: HAP1
characteristics.characteristic: cell_type
characteristics.text: 2020-11-18
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Genomic DNA was isolated from sorted cells using a DNA mini kit (Qiagen) with the lysis step occurring overnight at 56 o C for decrosslinking.The gene trap insertion sites were amplified using a LAM-PCR procedure as described (Blomen et al., 2015). Using a biotinylated primer in the gene trap cassette, single-stranded DNA (ssDNA) products were generated for 120 cycles, captured on magnetic beads, a pre-adenylated ssDNA linker ligated to the 3 end, and a final round of exponential amplificationusing a primer containing Illumina sequencing compatible overhangs at the end of the LTR andin the ssDNA linker.
characteristics.characteristic: description
characteristics.text: Netherlands
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Haploid genetic screen stained for Ikba levels (antibody:L35A5, Cell Signaling Technologies) in HAP1 cells high channel
characteristics.characteristic: title