name: E-MTAB-14576:CarSAV_infected
characteristics.text: 2025-05-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Agricultural Institute, HUN-REN Centre for Agricultural Research
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS21409944
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-14576:CarSAV_infected
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: clove pink
characteristics.characteristic: common name
characteristics.text: Tanga
characteristics.characteristic: cultivar
characteristics.text: Protocols: Samples were taken from CarSAV infected as well as from healthy culture for total RNA extraction.  Carnation (D. caryophyllus L. var. Tanga) plantlets were micropropagated and cultured based on the method Piquares and co-workers (2002). The extraction of small RNA and small RNA enrichment was carried out based on the method Cárdenas et al. (2011). For miRNA-Seq library construction, NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs, Ipswich, MA, USA) was used according to the manufacturer’s protocol. The quality and quantity of the library were determined by using a High Sensitivity DNA1000 ScreenTape system with 2200 Tapestation (Agilent Technologies, Santa Clara, CA, USA) and dsDNA HS Assay Kit with Qubit 3.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA).
characteristics.characteristic: description
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: CarSAV infection
characteristics.characteristic: infect
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Dianthus caryophyllus
characteristics.characteristic: organism
characteristics.text: Dianthus caryophyllus
characteristics.characteristic: organism
characteristics.text: portion of plant tissue
characteristics.characteristic: organism part
characteristics.text: Dianthus caryophyllus
characteristics.characteristic: scientific_name
characteristics.text: CarSAV_infected
characteristics.characteristic: title