name: E-MTAB-13861:Sample 2
characteristics.text: 2025-05-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Institute of Immunobiology, Kantonsspital St. Gallen, St. Gallen, Switzerland
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS18361576
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-13861:Sample 2
characteristics.characteristic: Submitter Id
characteristics.text: 59.8
characteristics.unit: year
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: mixed
characteristics.characteristic: cell_type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: After surgical excision torn tissues were collected sterile phosphate buffered saline on ice and processed within one hour of resection. Following the commercial brochure of Visium gene expression kits (10x Genomics, PN-1000184) the tissue blocks were cut into 10-μm sections using cryostat and mounted on Visium gene expression slides (10x Genomics, PN-1000188). Sections on Visium Gene Expression slides were first fixed with pre-cooled methanol for 30 minutes and stained with CD20, CD4, CD31 and aSMA and imaged with confocal microscopy to visualize the fiducial frames of capture areas and cell compartmentalization in different sections. The optimal permeabilization time for murine lymph node sections was 6 minutes evaluated by 10x Genomics Visium tissue optimization kit (10x Genomics, PN-1000193) for in situ hybridization and reverse transcription. cDNA libraries were generated using Visium spatial gene expression 3′ library construction kit (10x Genomics, PN-1000190).
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: warthin Tumor
characteristics.characteristic: disease
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: LN 06
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: lymph node
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: Sample 2
characteristics.characteristic: title