name: E-MTAB-14143:Sample 7
characteristics.text: 2025-05-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Division of Stem Cells and Cancer, German Cancer Research Center (DKFZ) and DKFZ-ZMBH Alliance, 69120 Heidelberg, Germany Heidelberg Institute for Stem Cell Technology and Experimental Medicine (HI-STEM gGmbH), 69120 Heidelberg, Germany
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS20182466
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-14143:Sample 7
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: AML samples were collected from diagnostic peripheral blood or bone marrow aspirations at the University Hospitals in Heidelberg or Hannover in accordance with the Declaration of Helsinki and based on institutional approvals after obtaining written informed consent from each patient. The project was approved by the Ethics Committee of the Medical Faculty of Heidelberg (S-169/2017 and S-648/2021) and the local Ethics Review Committee of Hannover Medical School (ethical vote No.7972_BO_K_2018). Mononuclear cells were isolated by density gradient centrifugation using Ficoll Paque Plus (GE Healthcare, cat #GE17-1440-03), and stored in liquid nitrogen until further use. Primary AML cells were stained with cell surface antibodies and sorted for live CD45+ cells in PBS/BSA. scRNA-seq library preparation was performed using the Chromium Single Cell 3’ Library and Gel Bead Kit (10X Genomics, cat # 1000128), according to manufacturer's instructions. Five to ten thousand cells were targeted for each sample, and processed according to the 10X Genomics Single Cell 3′ v3.1 protocol to generate the transcriptome libraries. The standard 10x Genomics Chromium Single Cell 3’ (v.3.1 chemistry) protocols were carried out according to manufacturer’s recommendations for the generation of scRNA-seq libraries.
characteristics.characteristic: description
characteristics.text: adult acute myeloid leukemia
characteristics.characteristic: disease
characteristics.text: Diagnosis
characteristics.characteristic: disease staging
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: P14
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: blood or bone marrow
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Sample 7
characteristics.characteristic: title