name: E-MTAB-15058:DMSO 2
characteristics.text: 2025-05-01
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Chair for Pharmaceutical and Medicinal Chemistry, Prof. Dr. Daniel Merk, Department Pharmazie, Ludwig-Maximilians-Universität München, Munich, Germany
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS24005472
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-15058:DMSO 2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: HEK293T
characteristics.characteristic: cell line
characteristics.text: epithelial
characteristics.characteristic: cell type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Samples were collected by aspirating stimulation medium and washing each well with PBS once. Thereafter cells were lysed using the lysis buffer from  E.Z.N.A. Total RNA Kit I (Omega Bio-tek) according to the manufacturer’s instructions. HEK293T cells were seeded at a density of 3 · 106 cells per well in 12-well plates and complete culture medium. After 24 h, medium was changed to DMEM high glucose supplemented with sodium pyruvate (1 mM), penicillin-streptomycin (100 U/mL and 100 µg/mL, respectively) and 0.2% FCS for synchronization over 24 h. Medium was changed again to DMEM high glucose supplemented with sodium pyruvate (1 mM), penicillin-streptomycin (100 U/mL and 100 µg/mL, respectively) and 0.2% FCS, additionally containing either test compounds  (1 µM) in 0.1% DMSO or 0.1% DMSO. After 17 h, cells were harvested the cells.  Total RNA was isolated using E.Z.N.A. Total RNA Kit I (Omega Bio-tek) according to the manufacturer’s instructions. RNA concentration and purity were assessed using a NanoDrop One UV–vis spectrophotometer (Thermo Fisher Scientific) at 260/280 nm. Novoegene repeated the isolation using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library. For the non-directional library, it was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. For the directional library, it was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection.
characteristics.characteristic: description
characteristics.text: embryo stage
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: adrenal cortex
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: DMSO 2
characteristics.characteristic: title