2025-05-01
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA113319745
External Id
Institut fur Pharmakologie Medizinische Fakultat der Heinrich-Heine-Universitat Dusseldorf
INSDC center name
2023-05-02T13:10:57Z
INSDC last update
public
INSDC status
ERS15314906
SRA accession
E-MTAB-12882:555-2
Submitter Id
12
week
age
ArrayExpress
broker name
cardiac interstitial cell
cell_type
house mouse
common name
Protocols: After mouse sacrifice hearts were excised and briefly washed in ice-cold PBS. Aorta was cannulated and coronaries retrograde flushed with 5 ml PBS/Heparin followed by 5 ml Collagenase/DNAse solution. Heart tissue was minced with scissors, transferred into 5ml Collagenase/DNAse solution and digested for 20 minutes at 37°C on a rotator. Cells were filtered over a 100µm and 40µm filter and pelleted at 800g, 4°C for 10 minutes. Cells were stained with a-TER antibody to identify erythrocytes and propidium iodide to identify dead cells. Cells were sorted and single, TER negative, PI negative cells were further used for sequencing. All used samples showed a cell vitality >80%. Chromium Single Cell 3’ NextGEM Reagent Kit v3.1 according to the manufacturer’s instructions Chromium Single Cell 3’ NextGEM Reagent Kit v3.1 according to the manufacturer’s instructions
description
adult
developmental stage
wild type genotype
genotype
752647
individual
Mus musculus
organism
Mus musculus
organism
heart
organism part
Mus musculus
scientific_name
male
sex
B6.129-Gt(ROSA)26Sortm1(CAG-CHRM4*,-mCitrine)Ute/J Tg(Adipoq-cre/ERT2)1Soff/J
strain
555-2
title