SAMN00008624
External Id
University of Notre Dame
INSDC center name
2010-03-18T00:00:00Z
INSDC first public
2025-04-29T21:07:48.527Z
INSDC last update
SRS010312
INSDC secondary accession
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
SRS010312
SRA accession
Adult females were collected from 4 populations of E. propertius, near the latitudinal center of the species distribution (within 50 km of Medford, Oregon). Eggs laid by these females were hatched under conditions characteristic of native habitats in a greenhouse and then moved to Conviron growth chambers at the University of Notre Dame. Multiple individuals of each larval instar were collected through the final instar before pupation. Individuals of the 2nd, 3rd, and 4th instars were exposed to a heat stress of 35 degrees for 60 minutes and a cold stress of 0 degrees for 120 minutes. Individuals in the 5th and 6th instar of were exposed to a desiccation agent (silica gel) for 120 minutes. After treatment, larvae were frozen in liquid nitrogen and stored at -80 degrees C. Whole-body RNA from these frozen individuals was extracted using an RNA Easy kit (QIAGEN Inc.) over a period of two months. Prior to library construction, pooling was done by adjusting sample contributions to equimolar amounts of total RNA per individual. In total, 53 larvae of were pooled prior to sequencing, representing a minimum of 11 and a maximum of 48 wild mothers.
description
Gesta propertius
http://purl.obolibrary.org/obo/NCBITaxon_3056699
organism
Generic sample from Erynnis propertius
title