2025-05-07
ENA first public
ERC000011
ENA-CHECKLIST
Duke-NUS Medical School
INSDC center name
public
INSDC status
ERS23715798
SRA accession
E-MTAB-14545:CVA062_3_P7
Submitter Id
49
year
age
ArrayExpress
broker name
1_haem
cancer_group
not collected
collection date
human
common name
Protocols: Whole blood was collected and stored at -80°C. Samples were incubated at room temperature for 2 hours prior to RNA extraction.  PAXgene Blood RNA Kit (Qiagen, 762174) was used to isolate RNA from blood. The NEBNext Globin and rRNA Depletion Kit (NEB, E7775X) was used to deplete globin and rRNA from 300ng of total RNA. NEBNext Ultra II RNA Library Prep Kit (NEB, E7775L) was used to construct the RNA libraries. HT DNA Extended Range LabChip (Perkin Elmer, 760517) was used to verify the library sizes and concentration, as well as pool to equal concentrations prior to sequencing.
description
adult
developmental stage
COVIDpos
disease
not collected
geographic location (country and/or sea)
CVA062
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
blood
organism part
Homo sapiens
scientific_name
female
sex
CVA062_3_P7
title