2025-05-07
ENA first public
ERC000011
ENA-CHECKLIST
Duke-NUS Medical School
INSDC center name
public
INSDC status
ERS23715832
SRA accession
E-MTAB-14545:CVA143_1_P7
Submitter Id
66
year
age
ArrayExpress
broker name
2_GI
cancer_group
not collected
collection date
human
common name
Protocols: Whole blood was collected and stored at -80°C. Samples were incubated at room temperature for 2 hours prior to RNA extraction.  PAXgene Blood RNA Kit (Qiagen, 762174) was used to isolate RNA from blood. The NEBNext Globin and rRNA Depletion Kit (NEB, E7775X) was used to deplete globin and rRNA from 300ng of total RNA. NEBNext Ultra II RNA Library Prep Kit (NEB, E7775L) was used to construct the RNA libraries. HT DNA Extended Range LabChip (Perkin Elmer, 760517) was used to verify the library sizes and concentration, as well as pool to equal concentrations prior to sequencing.
description
adult
developmental stage
COVIDneg
disease
not collected
geographic location (country and/or sea)
CVA143
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
blood
organism part
Homo sapiens
scientific_name
female
sex
CVA143_1_P7
title