2025-05-09
ENA first public
ERC000011
ENA-CHECKLIST
EMBL
INSDC center name
public
INSDC status
ERS20058519
SRA accession
E-MTAB-14105:S_LFL4_GFP_pos_8638
Submitter Id
ArrayExpress
broker name
limb bud mesenchyme cell
cell_type
not collected
collection date
house mouse
common name
Protocols: The distal region of a limb bud was isolated from Sox9-EGFP mouse embryos. Ectoderm was removed. The isolated mesenchyme cells were further dissociated by pipetting into a single-cell suspension and sorted with FACS Aria (BD) for the Sox9-EGFP signal-positive or signal-negative populations. Total RNA was extracted from each of the sorted populations. cDNA library were prepared from 10 ng of total RNA with SMARTer Ultra Low Input RNA Kit for Sequencing v3 (Clontech) and NEBNext Ultra DNA Library Prep Kit for Illumina according to the manufacturer's protocols.
description
mE11:20 (corresponding morphological stage of the hindlimb)
developmental stage
Sox9-positive
genotype
not collected
geographic location (country and/or sea)
Embryo 4
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
Forelimb autopod
organism part
Mus musculus
scientific_name
Sox9-EGFP
strain
S_LFL4_GFP_pos_8638
title