2025-05-10
ENA first public
ERC000011
ENA-CHECKLIST
UOM-BCF
INSDC center name
public
INSDC status
ERS24141440
SRA accession
E-MTAB-15080:Sample 17
Submitter Id
ArrayExpress
broker name
not collected
collection date
Protocols: We used six C. elegans strains: N2(wild type), wdr-5(ok1417) III, rbbp-5(tm3463) II, ash-2(tm1905) II, and the double mutants  wdr-5(ok1417) III; rbbp-5(tm3463) II and ash-2(tm1905) II; wdr-5(ok1417) III. Strains were kept at 20°C. 20 x 10cm plates of gravid adults were bleached and synchronised L1s were seeded on 2 X Corning tissue culture dishes (245 x 245 mm) seede with 20x OP50. When the synchronised young gravid mothers started to lay eggs, they were bleached to obtained embryos. These embryos were scored to ensure comparative stages between wild type and the mutants. About 10-12% of embryos are at <28-cell stage, 25-30% of embryos are between the 28-cell stage and 100-cell stage, 45-55% are between ~100-cell stage and pre-coma stage (~400 cell), and 5-15% are post-coma stage. Three independent biological replicates were prepared for RNA-seq. Total RNA was extracted using TRIzol (Invitrogen) and samples were frozen at -80°C. First strand cDNA synthesis was performed using the SuperScript VILO cDNA Synthesis Kit (Invitrogen), according to the manufacturer's instructions. Illumina TruSeq Stranded mRNA Library Prep Kit
description
Drosophila embryo stage
developmental stage
wdr-5(-)
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Caenorhabditis elegans
organism
Caenorhabditis elegans
organism
embryoid body
organism part
Caenorhabditis elegans
scientific_name
wdr-5(ok1417) III
strain
Sample 17
title