2025-05-12
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA113427632
External Id
Shanghai Institute of Rheumatology, Renji Hospital, Shanghai Jiao Tong University School of Medicine (SJTUSM), Shanghai, China
INSDC center name
2023-05-12T09:17:38Z
INSDC last update
public
INSDC status
ERS15422758
SRA accession
E-MTAB-12905:CKO-1
Submitter Id
8-12
week
age
ArrayExpress
broker name
naive B cell
cell_type
house mouse
common name
Protocols: Splenic B cells from B-cell specific Zeb2-CKO mice and WT mice were purified by negative selection and were induced in the presence of R848, anti-CD40,anti-IgM F(ab’)2 , IL-21, IFN-γ for 3 days, then, cells were collected. Briefly, 50,000 live cells were freshly isolated and resuspended in lysis buffer (10 mM Tris·Cl, (pH 7.4) ,10 mM NaCl，3 mM MgCl2，0.1% (v/v) Igepal CA-630) and pelleted. The cell pellet was resuspended in transposase reaction mix (5×TTBL 10μl, TTE Mix V50 5μl, ddH2O 35μl) and incubated at 37°C for 30 min. Fragmented DNA was purified using VAHTS DNA Clean Beads and library was generated by PCR.
description
adult
developmental stage
B-cell specific Zeb2-CKO
genotype
Mus musculus
organism
Mus musculus
organism
Mus musculus
scientific_name
C57BL/6J
strain
CKO-1
title