SAMN48112982
External Id
Stockholm University
INSDC center name
2025-05-11T00:41:25.243Z
INSDC first public
2025-05-11T00:41:25.243Z
INSDC last update
SRS24830841
INSDC secondary accession
live
INSDC status
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
SRS24830841
SRA accession
8 weeks
age
2025-01-30
collection_date
50,000 nuclei were mixed with a modified nuclei buffer and reagents, incubated at 37C for 30 minutes, and then loaded into a BD Rhapsody Cartridge after adding sample buffer. Nuclei were sedimented, washed, and incubated with splint beads before being agitated and washed again. After lysis with DTT and proteinase K, tagmented DNA and mRNA were immobilized onto beads, followed by DNA ligation and reverse transcription using a mix containing dNTPs, RT buffer, and reverse transcriptase. For WTA library preparation, random priming and extension were performed, followed by purification with AMPure XP Beads. The WTA libraries were then amplified, indexed, and analyzed on a Bioanalyzer to verify DNA size and concentration.
design_description
Cre_scWTA_S7_L005_R1_001.fastq.gz
filename
Cre_scWTA_S7_L008_R1_001.fastq.gz
filename10
Cre_scWTA_S7_L008_R2_001.fastq.gz
filename11
Cre_scWTA_S7_L008_R3_001.fastq.gz
filename12
Cre_scWTA_S7_L005_R2_001.fastq.gz
filename2
Cre_scWTA_S7_L005_R3_001.fastq.gz
filename3
Cre_scWTA_S7_L006_R1_001.fastq.gz
filename4
Cre_scWTA_S7_L006_R2_001.fastq.gz
filename5
Cre_scWTA_S7_L006_R3_001.fastq.gz
filename6
Cre_scWTA_S7_L007_R1_001.fastq.gz
filename7
Cre_scWTA_S7_L007_R2_001.fastq.gz
filename8
Cre_scWTA_S7_L007_R3_001.fastq.gz
filename9
fastq
filetype
China
geo_loc_name
Illumina NovaSeq X Plus
instrument_model
Cre_scWTA_lib1
library_id
paired
library_layout
cDNA_randomPriming
library_selection
TRANSCRIPTOMIC SINGLE CELL
library_source
RNA-Seq
library_strategy
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
ILLUMINA
platform
male
sex
C57BL/6J
strain
Testis
tissue
Model organism or animal sample from Mus musculus
title
Mus musculus Testis Cre_single-cell RNA-seq
title