SAMN46311800
External Id
Universidade de Coimbra
INSDC center name
2025-05-09T00:00:00Z
INSDC first public
2025-05-09T06:42:01.923Z
INSDC last update
SRS23946814
INSDC secondary accession
live
INSDC status
Metagenome or environmental
NCBI submission model
Metagenome.environmental.1.0
NCBI submission package
SRS23946814
SRA accession
2020
collection_date
PacBio HiFi long-read sequencing was performed on a pool sample of integron amplicons obtained with the primers: intI1-R [GCGAACGARTGBCGVAGVGTGTG] +3CS[AAGCAGACTTGACCTGA] and intI3F [AGTGGGTGGCGAATGAGTG] + class3R [CGTCAAACGGGTAAGCAGT]. Twelve monthly samples were amplified with each primer and then pooled. Forward primers already had unique barcodes associated. Only one library preparation was performed (the barcodes inserted in library prep are: 5 end: CGCATGACACGTGTGTT; 3 end: AACACACGTGTCATGCG). The sample specific barcodes (are only present at the 5'end) are as follows: January_int1: ATCACG, February_int1: CGATGT, March_int1: TTAGGC, April_int1: TGACCA, May_int1: ACAGTG, June_int1: GCCAAT, July_int1: CAGATC, August_int1: ACTTGA, September_int1: GATCAG, October_int1: TAGCTT, November_int1: GGCTAC, December_int1: CTTGTA, January_int3: AGTCAA, February_int3: AGTTCC, March_int3: ATGTCA, April_int3: CCGTCC, May_int3: GTAGAG, June_int3: GTCCGC, July_int3: ACTGAT, August_int3: ATGAGC, September_int3: ATTCCT, October_int3: CAAAAG, November_int3: CAACTA, December_int3: CACCGG
description
Portugal
geo_loc_name
treated wastewater
isolation_source
not provided
lat_lon
metagenome
http://purl.obolibrary.org/obo/NCBITaxon_256318
organism
Pool sample of integron amplicon sequences
title