2025-05-12
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA113427636
External Id
Shanghai Institute of Rheumatology, Renji Hospital, Shanghai Jiao Tong University School of Medicine (SJTUSM), Shanghai, China
INSDC center name
2023-05-12T09:17:38Z
INSDC last update
public
INSDC status
ERS15422762
SRA accession
E-MTAB-12905:WT-2
Submitter Id
8-12
week
age
ArrayExpress
broker name
naive B cell
cell_type
house mouse
common name
Protocols: Splenic B cells from B-cell specific Zeb2-CKO mice and WT mice were purified by negative selection and were induced in the presence of R848, anti-CD40,anti-IgM F(ab’)2 , IL-21, IFN-γ for 3 days, then, cells were collected. Briefly, 50,000 live cells were freshly isolated and resuspended in lysis buffer (10 mM Tris·Cl, (pH 7.4) ,10 mM NaCl，3 mM MgCl2，0.1% (v/v) Igepal CA-630) and pelleted. The cell pellet was resuspended in transposase reaction mix (5×TTBL 10μl, TTE Mix V50 5μl, ddH2O 35μl) and incubated at 37°C for 30 min. Fragmented DNA was purified using VAHTS DNA Clean Beads and library was generated by PCR.
description
adult
developmental stage
wild type genotype
genotype
Mus musculus
organism
Mus musculus
organism
Mus musculus
scientific_name
C57BL/6J
strain
WT-2
title