SAMN48112973
External Id
Stockholm University
INSDC center name
2025-05-11T00:41:25.643Z
INSDC first public
2025-05-11T00:41:25.643Z
INSDC last update
SRS24830832
INSDC secondary accession
live
INSDC status
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
SRS24830832
SRA accession
11dpp
age
2025-01-30
collection_date
50,000 nuclei were mixed with a modified nuclei buffer and reagents, incubated at 37C for 30 minutes, and then loaded into a BD Rhapsody Cartridge after adding sample buffer. Nuclei were sedimented, washed, and incubated with splint beads before being agitated and washed again. After lysis with DTT and proteinase K, tagmented DNA and mRNA were immobilized onto beads, followed by DNA ligation and reverse transcription using a mix containing dNTPs, RT buffer, and reverse transcriptase. For WTA library preparation, random priming and extension were performed, followed by purification with AMPure XP Beads. The WTA libraries were then amplified, indexed, and analyzed on a Bioanalyzer to verify DNA size and concentration.
design_description
11dpp_scWTA_S3_L001_R1_001.fastq.gz
filename
11dpp_scWTA_S3_L005_R2_001.fastq.gz
filename10
11dpp_scWTA_S3_L001_R2_001.fastq.gz
filename2
11dpp_scWTA_S3_L002_R1_001.fastq.gz
filename3
11dpp_scWTA_S3_L002_R2_001.fastq.gz
filename4
11dpp_scWTA_S3_L003_R1_001.fastq.gz
filename5
11dpp_scWTA_S3_L003_R2_001.fastq.gz
filename6
11dpp_scWTA_S3_L004_R1_001.fastq.gz
filename7
11dpp_scWTA_S3_L004_R2_001.fastq.gz
filename8
11dpp_scWTA_S3_L005_R1_001.fastq.gz
filename9
fastq
filetype
China
geo_loc_name
Illumina NovaSeq X Plus
instrument_model
11dpp_scWTA_lib1
library_id
paired
library_layout
cDNA_randomPriming
library_selection
TRANSCRIPTOMIC SINGLE CELL
library_source
RNA-Seq
library_strategy
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
ILLUMINA
platform
male
sex
C57BL/6J
strain
Testis
tissue
Mus musculus Testis 11dpp single-cell RNA-seq
title
Model organism or animal sample from Mus musculus
title