SAMN48112977
External Id
Stockholm University
INSDC center name
2025-05-11T00:41:25.293Z
INSDC first public
2025-05-11T00:41:25.293Z
INSDC last update
SRS24830836
INSDC secondary accession
live
INSDC status
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
SRS24830836
SRA accession
17dpp
age
2025-01-29
collection_date
To tagment DNA, 50,000 nuclei were mixed with a modified nuclei buffer and reagents, incubated at 37C for 30 minutes, and then loaded into a BD Rhapsody Cartridge after adding sample buffer. Nuclei were sedimented, washed, and incubated with splint beads before being agitated and washed again. After lysis with DTT and proteinase K, tagmented DNA and mRNA were immobilized onto beads, followed by DNA ligation and reverse transcription using a mix containing dNTPs, RT buffer, and reverse transcriptase. Unused oligos were removed via exonuclease treatment, and DNA was eluted twice for ATAC-seq library preparation. The ATAC-seq libraries were then amplified and indexed using PCR.
design_description
17dpp_scATAC_S2_L001_R1_001.fastq.gz
filename
17dpp_scATAC_S2_L004_R1_001.fastq.gz
filename10
17dpp_scATAC_S2_L004_R2_001.fastq.gz
filename11
17dpp_scATAC_S2_L004_R3_001.fastq.gz
filename12
17dpp_scATAC_S2_L001_R2_001.fastq.gz
filename2
17dpp_scATAC_S2_L001_R3_001.fastq.gz
filename3
17dpp_scATAC_S2_L002_R1_001.fastq.gz
filename4
17dpp_scATAC_S2_L002_R2_001.fastq.gz
filename5
17dpp_scATAC_S2_L002_R3_001.fastq.gz
filename6
17dpp_scATAC_S2_L003_R1_001.fastq.gz
filename7
17dpp_scATAC_S2_L003_R2_001.fastq.gz
filename8
17dpp_scATAC_S2_L003_R3_001.fastq.gz
filename9
fastq
filetype
China
geo_loc_name
Illumina NovaSeq X Plus
instrument_model
17dpp_scATAC_lib1
library_id
paired
library_layout
PCR
library_selection
GENOMIC SINGLE CELL
library_source
ATAC-seq
library_strategy
Mus musculus
http://purl.obolibrary.org/obo/NCBITaxon_10090
organism
ILLUMINA
platform
male
sex
C57BL/6J
strain
Testis
tissue
Model organism or animal sample from Mus musculus
title
Mus musculus Testis 17dpp single-cell ATAC-seq
title