2025-05-12
ENA first public
ERC000011
ENA-CHECKLIST
Instituto Nacional de Perinatología Isidro Espinosa de los Reyes
INSDC center name
public
INSDC status
ERS24422317
SRA accession
E-MTAB-15111:Sample 5
Submitter Id
3
month
age
ArrayExpress
broker name
not collected
collection date
Norway rat
common name
Protocols: At embryo day 12, pregnant rats were euthanized by decapitation following anesthesia induction with sevoflurane; embryos were collected by cesarian and washed in cold phosphate-buffered saline (PBS, pH 7.4). The hole prosencephalon of embryos without neural tube defects was directed and placed in Trizol for RNA extraction. Embryos of both sexes were used without distinction in all experiments.  Total RNA was obtained using TRIZOL® reagent (Thermo Fisher Scientific, MA, USA) following provider’s instructions. RNA quality and purity were determined (Nanodrop, Thermo Fisher Scientific), and only 260/230 ≥ 1.0 and 260/280 ≥ 1.8 samples were used. RNA Integrity Number (RIN) was obtained by using a BioAnalyzer (Agilent 2100 BioAnalyzer, CA, USA). The mean RIN was 9.04 ± 0.44 RNA-seq libraries were prepared using the Truseq Stranded mRNA library prep kit from (Illumina, CA, USA) according to the manufacturer’s instructions.
description
twelve days of embryonic development
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
Diabetic
individual
not applicable
isolate
Rattus norvegicus
organism
Rattus norvegicus
organism
Prosencephalon
organism part
Rattus norvegicus
scientific_name
mixed
sex
Wistar
strain
Sample 5
title