2025-05-12
ENA first public
ERC000011
ENA-CHECKLIST
Instituto Nacional de Perinatología
INSDC center name
public
INSDC status
ERS24441095
SRA accession
E-MTAB-15128:120h_NAc_FeCo_2
Submitter Id
three
month
age
ArrayExpress
broker name
not collected
collection date
prairie vole
common name
Protocols: Subjects were euthanized with an overdose of pentobarbital (6.3 mg/vol) by intraperitoneal (IP) administration, and the whole brains were obtained to dissect the SVZ, DG, and NAc tissues. Briefly, whole brains were collected in Petri dishes with an ice-cold solution of 1X PBS, 2 mM HEPES, 20 mM glucose, and 25 mM NaHCO3. The three brain regions were dissected under a stereoscope and immediately frozen. Total RNA was isolated from each sample using TRIzol and DNase I.RNA integrity was assessed using a Agilent BioAnalyzer, retaining only samples with an RNA integrity number (RIN) >8.0.  Libraries were constructed using the Truseq Stranded mRNA library prep kit  according to the manufacturer’s instruction (Illumina).
description
adult
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
Pool of three individuals
individual
not applicable
isolate
Microtus ochrogaster
organism
Microtus ochrogaster
organism
brain
organism part
Microtus ochrogaster
scientific_name
female
sex
Control (isolated individual)
sociosexual condition
Not determined
strain
120h_NAc_FeCo_2
title