2025-05-12
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA113448283
External Id
Shanghai Institute of Rheumatology, Renji Hospital, Shanghai Jiao Tong University School of Medicine (SJTUSM), Shanghai, China
INSDC center name
2023-05-16T16:19:47Z
INSDC last update
public
INSDC status
ERS15443049
SRA accession
E-MTAB-12903:Ctrl
Submitter Id
8 to 12
week
age
ArrayExpress
broker name
B cell
cell_type
house mouse
common name
Protocols: bm12-cGVHD lupus model was induced following JOVE protocol. Briefly, 8-12-week-old mice were intraperitoneally injected with 10 million CD4+ T cells from bm12 mice purified by magnetic cell sorting and mice were sacrificed for analysis after 2 weeks. Single cell suspensions of splenocytes from Bm12-induced lupus T-bet reporter mice were subjected to red blood cell lysis and filtered through 70 um nylon mesh.Cells were stained for surface markers under instructions. DP and DN B cells were collected through flow cytometry. Briefly, 50,000 live cells were freshly isolated and resuspended in lysis buffer (10 mM Tris·Cl, (pH 7.4) ,10 mM NaCl，3 mM MgCl2，0.1% (v/v) Igepal CA-630) and pelleted. The cell pellet was resuspended in transposase reaction mix (5×TTBL 10μl, TTE Mix V50 5μl, ddH2O 35μl) and incubated at 37°C for 30 min. Fragmented DNA was purified using VAHTS DNA Clean Beads and library was generated by PCR.
description
CD19 positive
immunophenotype
Mus musculus
organism
Mus musculus
organism
spleen
organism part
Mus musculus
scientific_name
female
sex
C57BL/6J
strain
Ctrl
title