2025-05-16
ENA first public
ERC000011
ENA-CHECKLIST
POSTECH
INSDC center name
public
INSDC status
ERS24142050
SRA accession
E-MTAB-15083:WT_F1_pollen2
Submitter Id
ArrayExpress
broker name
not collected
collection date
thale cress
common name
Col/Ler F1
cultivar
Protocols: To purify pollen grains from F1 hybrid plants and extract their genomic DNA, whole inflorescences of hybrid plants were collected and transferred to ice-cold 10% (w/v) sucrose in a 50-ml tubes. Flowers were homogenized with a blender (Tefal, BL3051KR), filtered through 80-μm nylon mesh, and centrifuged at 350g for 10 min at 4 °C. The supernatant was discarded and the pellet was resuspended in 10% (w/v) sucrose, filtered through a 40-μm cell strainer, and centrifuged at 100g for 10 min at 4°C. The purified pellet was frozen in liquid nitrogen. long day conditions (16 hr light), 20 degrees Celcius DNA was extracted using the CTAB method. Nanobind disk (Nanobind Plant Nuclei Kit RT, PacBIO, 102-302-000) was used to collect high molecular weight genomic DNA. Ligation Sequencing Kit V14 (Nanopore, SQK-LSK114)
description
plant structure development stage
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Arabidopsis thaliana
organism
Arabidopsis thaliana
organism
pollen
organism part
Arabidopsis thaliana
scientific_name
WT_F1_pollen2
title