SAMN03152044
External Id
New York University
INSDC center name
2015-03-01T00:00:00Z
INSDC first public
2025-05-13T14:03:32.611Z
INSDC last update
SRS733540
INSDC secondary accession
live
INSDC status
Plant
NCBI submission model
Plant.1.0
NCBI submission package
SRS733540
SRA accession
3 weeks old
http://purl.obolibrary.org/obo/HsapDv_0000025
age
Coruzzi lab, New York University
biomaterial provider
missing
collection_date
Columbia
http://www.ebi.ac.uk/efo/EFO_0005147
ecotype
WT and sdg8-5 (previously named cli186) seeds were surface sterilized and imbibed in darkness for 2 days. Plants were then grown hydroponically inside a sterile Phytatray (Sigma-Aldrich, St. Louis, MO, USA) on liquid Basal MS medium (GIBCO Formula# 97-5068EC) supplemented with 1% sucrose and 2 mM KNO3 at a pH of 5.7. The phytatrays were kept under white light (50 uE m-2s-1) in long-day cycle (16h Light/8h Dark) at 220C for 3 weeks (when they start flowering). After 3 weeks, the plants were starved in liquid basal MS medium (GIBCO Formula# 97-5068EC) supplemented with 0% sucrose and 2 mM KNO3 at a pH of 5.7 and the phytatrays were covered with aluminum foil (for light starvation) for 24 hours. Plants were then treated with +/- 1% Sucrose and +/- Light (70 uEin m-2s-1) for 2 hours.
growth_protocol
Arabidopsis thaliana
http://purl.obolibrary.org/obo/NCBITaxon_3702
organism
shoots
http://purl.obolibrary.org/obo/PO_0009006
tissue
Plant shoots sample from Arabidopsis thaliana
title